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Your present location:Home/Products/DNA&RNA Purification/DNA/Circulating DNA/Column Kits/HiPure Circulating Nucleic Acid Micro Kit
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HiPure Circulating Nucleic Acid Micro Kit

D3180
CAT NO PRODUCT NAME SIZE PRICE
D318002 HiPure Circulating Nucleic acid Micro Kit 50 preps $199.00
D318003 HiPure Circulating Nucleic acid Micro Kit 250 preps $895.00

Introduction

Free-circulating nucleic acids, such as tumor-specific extracellular nucleic acid fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp(Nucleic Acid). The HiPure Circulating Nucleic acid Micro Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be either fresh or frozen (provided that they have not been frozen and thawed more than once). Free-circulating cell-free DNA, RNA or viral nucleic acids are eluted in Nuclease Free Water, ready for use in amplification reactions or storage at -30 to -15°C. Purified nucleic acids are free of proteins, nucleases, and other impurities.

Details


Specifications

Features Specifications
Main Functions Isolation circulating DNA from 0.6ml plasma,serum, body fluids
Applications qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Serum, plasma and other cell-free fluid samples
Sample amount 0.6ml
Elution volume ≥30μl
Time per run ≤40 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg


Principle

This product is based onsilica column purification. The sample is lysed and digested with lysate andprotease, DNA is released into the lysate. Transfer to an adsorption plate andfilter column. Nucleic acid is adsorbed on the membrane, while protein is notadsorbed and is removed with filtration. After washing proteins and otherimpurities, Nucleic acid was finally eluted with low-salt buffer.

Advantages

  • High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
  • High concentration - low elution volume, ensuring high nucleic acid concentration
  • High purity - low alcohol binding method, completely removing inhibitor and protein pollution
  • High recovery - DNA can be recoveredat the level of PG by silica gel column purification


Kit Contents

Contents D318002 D318003
Purification Times 50 Preps 250 Preps
Buffer ACL 40 ml 200 ml
Buffer DCW1 22 ml 110 ml
Buffer DCW2* 20 ml 2 x 50 ml
Proteinase K 34 mg 180 mg
Protease Dissolve Buffer 1.8 ml 10 ml
Carrier RNA 110 μg 310 μg
Nuclease Free Water
10 ml 30 ml
HiPure CFDNA Mini Columns
50 250
2 ml Collection Tubes
100 5 x 100


Storage and Stability

Proteinase K, Carrier RNAshould be stored at 2-8°C upon arrival. However, short-term storage (up to 12weeks) at room temperature (15-25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15-25°C) andare stable for at least 18 months under these conditions. The entire kit can bestored at 2-8°C, but in this case buffers should be redissolved before use.Make sure that all buffers are at room temperature when used.

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