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MagPure Circulating DNA Rich Maxi Kit 12927

CAT NO PRODUCT NAME SIZE PRICE
1292750 MagPure Circulating DNA Rich Maxi Kit 50  preps $1094.00
12927200 MagPure Circulating DNA Rich Maxi Kit
200 preps $3978.00




Introduction

This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.

Details


Specifications

Features Specifications
Main Functions Isolation circulatingDNA from 5ml plasma, serum, body fluids
Applications qPCR, NGS, etc.
Purification technology Magnetic beads technology
Process method Manual (centrifugation or vacuum)
Sample type Serum, plasma
Sample amount 5ml
Elution volume ≥40μl
Time per run ≤50 minutes


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

  • Economy - less than 50% of the price of Qiagen and other imported products
  • Automatic - without labour


Kit Contents

Contents 1292750 12927200
Purification Times 50 200
MagPure Particles G
80 ml
Magbind Particles (selection particles)
58 ml
Selection Solution
400 ml
Proteinase K
1.2 g
Protease Dissolve Buffer
100 ml
Buffer SDS(20%)
60 ml
Buffer MLK

3 x 450 ml
Buffer BST1
2x 450 ml
Buffer MKW1

2x 450 ml
Buffer MW2

2x 100 ml
Buffer AE

30 ml

Storage and Stability

After receive the kit, store the MagPure Particles G, MagBind Particles and Proteinase K at 4°C. Other kit components can be stored at room temperature (15−25°C). Dissolved Proteinase K should be store at -20°C. The reagents are stable for up to one year from the manufacturing date.

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