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MagPure Plant RNA Kit

R6641
CAT NO PRODUCT NAME SIZE PRICE
R664101 MagPure Plant RNA Kit 48 preps $298.00
R664102 MagPure Plant RNA Kit 96 preps $497.00
R664103
MagPure Plant RNA Kit
480 preps
$1,999.00

Introduction

This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5x106 cultured cells, 20mg tissue and <50mg plant samples.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA from 50mg plant using magnetic particles
Applications RT-PCR, cDNA synthesis, second generation sequencing
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Plant and fungus samples
Sample amount
50mg
Yield 2-100μg
Time per run
60 minutes


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.


Advantages

  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Safe - no phenol chloroform extraction required
  • Fast - several samples can be extracted in 60 minutes by column method
  • Universal - two lysates suitable for most plant or fungal tissue samples


Kit Contents

Contents R664101 R664102 R664103
Purification Times 48 Preps 96 Preps
480 Preps
MagPure RNA Particles 1.7 ml 3.5 ml
18 ml
DNase I 600 μl 2 x 600 μl
10 x 600 μl
DNase Buffer
30 ml
40 ml
200 ml
Buffer PRC1
40 ml
70 ml
350 ml
Buffer RL 40 ml 70 ml
350 ml
Buffer MCB*
18 ml
30 ml
150 ml
Buffer MW1*
22 ml
44 ml
220 ml
Buffer MW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
15 ml
120 ml


Storage and Stability

MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.

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