HiPure Gel DNA Micro Kit
D2110
CAT NO | PRODUCT NAME | SIZE | PRICE |
D212102 | HiPure PCR Pure Mini Kit | 100 preps | $60.00 |
D212103 | HiPure PCR Pure Mini Kit | 250 preps | $150.00 |
Specifications
Features | Specifications |
Main Functions | Recover DNA fragments between 60bp-20kbp from PCR products and enzymatic reaction solution |
Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | PCR products, enzymatic reaction solution |
Sample amount |
10-200μl |
Recovery | ≥80% |
Elution volume | ≥15μl |
Time per run | ≤10 minutes |
Liquid carrying volume per column |
800µl |
Binding yield of column | 35µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
Contents | D212102 | D212103 |
Purification Times | 100 Preps |
250 Preps |
Buffer DP | 60 ml |
120 ml |
Buffer DW2 | 20 ml |
50 ml |
Elution Buffer |
15 ml |
30 ml |
HiPure DNA Mini Columns II |
100 |
250 |
2 ml Collection Tubes | 100 |
250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Experiment Data
D3018
IVD5412
IVD4173
IVD3020
R4011
D6364
D3141
D3146
D6351
D3187
D3125
D3126
IVD5432
D3182C
IVD3102
D3111