Introduction

The HiPure Fastfilter Plasmid DNA Midi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes aspecial filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 250 µg high copy number plasmid DNA or 10-75 µg low copy number plasmid DNA can be purified from 15-50 mL overnight culture.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 250μg plasmid DNA from 30-50ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling, etc.
Purification method	Midi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	30-50ml
Yield	50-250µg
Elution volume	≥300μl
Time per run	≤60 minutes
Liquid carrying volume per column	4ml
Binding yield of column	250µg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparationand clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast - it takes only 15-60 minutes to complete the isolation
High yield - up to 70µg plasmid can be binded in one column

Kit Contents

Contents	P101302	P101303
Purification Times	25 Preps	100 Preps
RNase A	10 mg	40 mg
Buffer P1	80 ml	300 ml
Buffer P2	80 ml	300 ml
Buffer LEN3	40 ml	150 ml
Buffer GBT	60 ml	250 ml
Buffer PW1	60 ml	250 ml
Buffer PW2	20 ml	100 ml
Elution Buffer	30 ml	120 ml
HiPure DNA Midi Columns III	25	100
Lysate Clear Midi Syringe	25	100
15 ml Collection Tubes	50	200

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

Name	CAT NO	Sample amount	Column type	Elutio volume	Yield	Time per run	Centrifuge requirements
HiPure Plasmid Mini Kit	P1001	1-5ml	1.5ml column	30-50μl	5–35µg/5ml	≤30 minutes	small centrifuge
HiPure Plasmid Mini Kit II	P1002	5-15ml	1.5ml column	75-100μl	20-80µg/15ml	≤30 minutes	small centrifuge
HiPure Plasmid Plus 96 Kit	P1006	1-1.5ml	96 well plate	70-150μl	1-15μg/1ml	≤60 minutes	96 well plate centrifuge
HiPure Fastfilter Plasmid Midi Kit	P1013	30-50ml	15ml column	0.3-0.8ml	50–250µg/50ml	≤60 minutes	15ml centrifuge
HiPure Fastfilter Plasmid Maxi Kit	P1014	100-200ml	50ml column	0.5-1.5ml	0.4–1mg/200ml	≤60 minutes	50ml centrifuge

Articles

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P1013 HiPure FastFilter Plasmid Midi Kit 2022.pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
P101302	HiPure Fastfilter Plasmid Midi Kit	25 preps	$249.00
P101303	HiPure Fastfilter Plasmid Midi Kit	100 preps	$895.00

HiPure Fastfilter Plasmid Midi Kit

Introduction

Details

Principle

Purchase Guide

Articles

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