Introduction

The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen's innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture
Applications	Cell transfection, animal injection, etc.
Purification method	Maxi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, large plasmid
Sample amount	High copy plasmid: 100-150ml culture medium Low copy plasmid: 150-200ml culture medium
Yield	100-1500μg
Elution volume	≥0.8ml
Time per run	≤60 minutes
Liquid carrying volume per column	20ml
Binding yield of column	1mg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast - silica gel column purification is much faster than ion exchange
High yield - up to 1.5mg plasmid can be binded in one column
Ultra low endotoxin - content of endotoxin is less than 0.05 EU/μg, which can be directly used for cell transfection and animal injection, etc.

Kit Contents

Contents	P115602	P115603
Purification Times	10 Preps	50 Preps
RNase A	30 mg	150 mg
Buffer P1	100 ml	500 ml
Buffer P2	100 ml	500 ml
Buffer LN3	50 ml	250 ml
Buffer PW1	33 ml	180 ml
Buffer PW2	20 ml	100 ml
Elution Buffer	15 ml	120 ml
Buffer CL	33 ml	180 ml
HiPure DNA Maxi Columns C	10	50
Lysate Clear Midi Syringe	10	50
50 ml Collection Tubes C	20	100

Storage and Stability

The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2–8°C.

Purchase Guide

Name	CAT NO	Sample amount	Column type	Elution volume	Yield	Time per run	Centrifuge requirements
HiPure Plasmid EF Mini Kit	P1154	10-15ml	1.5ml column	≥75μl	10–70µg/15ml	≤40 minutes	small centrifuge
HiPure Plasmid EF 96 Kit	P1157	1-5ml	96 well plate	≥75μl	30µg/1ml	≤60 minutes	96 well plate centrifuge
HiPure Plasmid EF Midi Kit	P1231	30-50ml	15ml column	≥0.4ml	10-500μg/50ml	≤60 minutes	15ml centrifuge
HiPure Plasmid EF Maxi Kit	P1156	100-200ml	50ml column	≥0.8ml	100–1500µg/200ml	≤60 minutes	50ml centrifuge
HiPure Plasmid EF Mega Kit	P1116	500ml	50ml column	≥0.8ml	10mg/500ml	≤70 minutes	50ml centrifuge

Articles

Download

P1156 HiPure Plasmid EF Maxi Kit 2022.pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
P115602	HiPure Plasmid EF Maxi Kit	10 preps	$164.00
P115603	HiPure Plasmid EF Maxi Kit	50 preps	$746.00

HiPure Plasmid EF Maxi Kit

Introduction

Details

Principle

Purchase Guide

Articles

Download

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