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MagPure Stool DNA LQ Kit D6364

CAT NO PRODUCT NAME SIZE PRICE
D636401 MagPure Stool DNA Kit 48 preps $239.00
D636402 MagPure Stool DNA Kit 96 preps $448.00
D636403
MagPure Stool DNA Kit
480 preps $2,138.00

Introduction

MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from 100-150mg stool samples
Applications PCR, southern blot and enzyme digestion, etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Stool
Sample amount 100-150 mg
Elution volume
Time per run


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.


Advantages

  • Fast - several samples can be extracted in 60 minutes (after digestion)
  • High purity - unique adsorbent can completely remove inhibitory factors
  • High recovery - DNA can be recovered at the level of PG


Kit Contents

Contents D636401
D636402 D636403
Purification Times 48 Preps
96 Preps 5 x 96 Preps
MagPure Particles
1.7 ml 3.5 ml
18 ml
2ml Bead Tubes
48 96
500
RNase A
10 mg
20 mg
100 mg
Proteinase K
24 mg
48 mg
200 mg
Protease Dissolve Buffer
3 ml 5 ml
20 ml
Buffer SPL
40 ml 80 ml
350 ml
Buffer PCI
40 ml 80 ml
350 ml
Buffer MLE
30 ml 60 ml
240 ml
Buffer GW1*
22 ml
44 ml
220 ml
Elution Buffer
20 ml
30 ml
120 ml


Storage and Stability

MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.