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Your present location:Home/Products/DNA&RNA Purification/DNA/Microbial DNA/Magnetic Kits/MagPure Soil DNA Kit
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MagPure Soil DNA Kit

D6356
CAT NO PRODUCT NAME SIZE PRICE
D635601 MagPure Soil DNA Kit 48 preps $239.00
D635602 MagPure Soil DNA Kit 96 preps $448.00
D635603
MagPure Soil DNA Kit
480 preps $2,138.00

Introduction

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from 250-500mg soil sample
Applications PCR, southern blot and enzyme digestion, etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Soil
Sample amount 250-500mg
Elution volume 50μl
Time per run 70 minutes


Principle

This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.


Advantages

  • High concentration - maximum extraction of total DNA from soil samples
  • High purity - purified DNA can be directly used in various downstream applications


Kit Contents

Contents D635601
D635602 D635603
Purification Times 48 Preps
96 Preps 5 x 96 Preps
MagPure Particles
2.5 ml 5 ml
22 ml
2ml Bead Tubes
48 96
400
Buffer SOL
60 ml 100 ml
500 ml
Buffer SDS
6 ml
10 ml
50 ml
Reagent DX
1 ml 1 ml 5 ml
Buffer PS
10 ml 20 ml
90 ml
Absorber Solution
10 ml 20 ml
90 ml
Buffer GDP
70 ml 150 ml
2 x 350 ml
Buffer GW1*
22 ml
44 ml
220 ml
Elution Buffer
20 ml
20 ml
60 ml


Storage and Stability

MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.


Experiment Data


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