| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D316302 | HiPure HP Plant DNA Maxi Kit | 10 preps | $224.00 |
| D316303 | HiPure HP Plant DNA Maxi Kit | 50 preps | $995.00 |
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 3g plant and fungal tissue |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Various plant samples (including conventional, polysaccharides and polyphenols) |
| Sample amount |
Fresh / frozen plant samples: 2-3 g Dried plant / seed samples: 0.5-1 g |
| Elution volume | ≥500μl |
| Time per run | ≤120 minutes |
| Liquid carrying volume per column | 20ml |
| Binding yield of column | 5mg |
This product is based on silica Column purification. Plant material is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged.DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Kit Contents
| Contents | D316302 | D316303 |
| Purification Times | 10 Preps | 50 Preps |
|
RNase A |
20 mg |
90 mg |
|
Protease Dissolve Buffer |
1.8 ml |
10 ml |
| Buffer PAL | 180 ml | 900 ml |
| Buffer GWP | 150 ml | 800 ml |
| Buffer GW2 | 25 ml | 200 ml |
|
Buffer AE |
30 ml |
120 ml |
|
HiPure DNA Maxi Columns II |
10 |
50 |
|
50 ml Collection Tubes |
20 |
100 |
Storage and Stability
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
| Name | CAT NO |
Fresh / frozen tissue amount |
Dried tissue amount |
Column type |
Elution volume |
Yield (muscle) |
| HiPure Plant DNA Mini Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 - 100μl | 3-70μg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 - 3ml | 75-1250μg |
|
HiPure SF Plant DNA Kit |
D3164 |
100mg |
20mg |
1.5ml column |
50 - 100μl |
3-50μg |
|
HiPure SF Plant DNA 96 Kit |
D3167 |
50mg |
15mg |
96 well plate |
75 - 150μl |
3-30μg |
Select the right purification kit to get impactful results：
Ⅰ. Purchase guide of Magen kits based on sample
Ⅱ. Cross purchase guide for Magen kits vs Other brands
For any customized product or OEM service, please contact us.
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