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MagPure Bacterial DNA Kit

D5131
CAT NO PRODUCT NAME SIZE PRICE
D513101 MagPure Bacterial DNA Kit 48 preps $174.00
D513102 MagPure Bacterial DNA Kit 96 preps $298.00
D513103
MagPure Bacterial DNA Kit
480 preps $1,293.00

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from bacterial culture
Applications PCR, southern blot and enzyme digestion, etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Gram negative or positive bacteria
Sample amount Bacterial culture: 0.5-1.5ml
Elution volume 60μl
Time per run 60 minutes


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.


Advantages

  • Fast - several samples can be extracted in 60 minutes (after digestion)
  • High purity - unique adsorbent can completely remove inhibitory factors
  • High recovery - DNA can be recovered at the level of PG


Kit Contents

Contents D513101
D513102 D513103
Purification Times 48 Preps
96 Preps 480 Preps
MagPure Particles
1.2 ml 2.5 ml
11 ml
Lysozyme
60 mg 120 mg
600 mg
Proteinase K
24 mg
50 mg
220 mg
Protease Dissolve Buffer
3 ml 6 ml
30 ml
Buffer SDS
1.5 ml 3 ml
15 ml
Buffer MLA
30 ml 60 ml
300 ml
Buffer GW1*
13 ml 44 ml
110 ml
Buffer TE
30 ml 40 ml
200 ml


Storage and Stability

MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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