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HiPure HP Plant DNA Maxi Kit

D3163
CAT NO PRODUCT NAME SIZE PRICE
D316302 HiPure HP Plant DNA Maxi Kit 10 preps $224.00
D316303 HiPure HP Plant DNA Maxi Kit 50 preps $995.00

Introduction

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from 3g plant and fungal tissue
Applications PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Various plant samples (including conventional, polysaccharides and polyphenols)
Sample amount

Fresh / frozen plant samples: 2-3 g

Dried plant / seed samples: 0.5-1 g

Elution volume ≥500μl
Time per run 120 minutes
Liquid carrying volume per column 20ml
Binding yield of column 5mg


Principle

This product is based on silica Column purification. Plant material is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged.DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages

  • Broad spectrum - suitable for extracting DNA from various plant samples
  • Fast - several samples can be extracted in 30 minutes by silica technology
  • High purity - high quality DNA, completely remove inhibitors
  • High yield - silica technology can achieve the highest yield


Kit Contents

Contents D316302 D316303
Purification Times 10 Preps 50 Preps
RNase A
20 mg
90 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer PAL 180 ml 900 ml
Buffer GWP 150 ml 800 ml
Buffer GW2 25 ml 200 ml
Buffer AE
30 ml
120 ml
HiPure DNA Maxi Columns II
10 50
50 ml Collection Tubes
20 100


Storage and Stability

RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Purchase Guide

Name CAT NO

Fresh / frozen tissue amount

Dried tissue amount

Column type

Elution volume

Yield (muscle)

HiPure Plant DNA Mini Kit D3187 150mg 40mg 1.5ml column 50 - 100μl 3-70μg
HiPure HP Plant DNA Maxi Kit D3163 5g 1g 50ml column 0.7 - 3ml 75-1250μg
HiPure SF Plant DNA Kit
D3164
100mg
20mg
1.5ml column
50 - 100μl
3-50μg
HiPure SF Plant DNA 96 Kit
D3167
50mg
15mg
96 well plate
75 - 150μl 3-30μg

Select the right purification kit to get impactful results

Ⅰ. Purchase guide of Magen kits based on sample

Ⅱ. Cross purchase guide for Magen kits vs Other brands

For any customized product or OEM service, please contact us.

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