Introduction
MagPure FFPE DNA/RNA Kit (R6327) represents the magnetic bead co-extraction configuration within the Magen FFPE nucleic acid purification portfolio. The system is designed to recover both genomic DNA and RNA from formalin-fixed paraffin-embedded tissue samples using a magnetic adsorption workflow.
Following tissue digestion, nucleic acids are separated through magnetic bead binding behavior, allowing DNA and RNA fractions to be isolated during the adsorption stage. This configuration supports efficient recovery of fragmented nucleic acids typically present in FFPE samples and is optimized for Higher total DNA and RNA yield.
Magnetic particle chemistry and bead-to-lysate ratios were refined through internal development cycles to improve adsorption efficiency and recovery stability across different FFPE tissue conditions.
Laboratories prioritizing RNA integrity during extraction may refer to MagPure FFPE DNA/RNA Kit – IVD3026, where nucleic acid partitioning occurs earlier during the digestion stage.
Column-based purification workflows are available through HiPure FFPE DNA Kit – D3126 for genomic DNA extraction and HiPure FFPE RNA Kit – R4143 for RNA purification.
R6327 functions as the High-recovery magnetic reference workflow within the Magen FFPE extraction system.
Details
Specifications
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Features
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Specifications
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Main Functions
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Co-isolation DNA and RNA from FFPE tissue
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Applications
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RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
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Purification method
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Polydisperse silicon based magnetic beads (DNA)
Monodisperse carbonyl magnetic beads (RNA)
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Adaptive instrument
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Nucleic acid extractor and pipetting workstation
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Sample type
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FFPE slice, FFPE puncture sample, embedded tissue
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Sample amount
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No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area
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Yield
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DNA: 1 - 10 μg, RNA: 1 - 25 μg
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Extraction Principle
Formalin fixation introduces crosslinks between nucleic acids and proteins, while paraffin embedding introduces hydrophobic contaminants that complicate nucleic acid recovery. In addition, nucleic acids extracted from FFPE tissues are typically fragmented due to fixation and long-term storage.
In the MagPure FFPE DNA/RNA workflow, tissue sections are first deparaffinized and digested with Proteinase K to release nucleic acids from the FFPE tissue matrix. A high-temperature incubation step is included to partially reverse formaldehyde crosslinking, improving accessibility of both DNA and RNA molecules.
Unlike conventional nucleic acid purification workflows where DNA or RNA is extracted separately, the MagPure FFPE DNA/RNA Kit uses a sequential magnetic adsorption strategy to isolate both nucleic acid types from the same lysate.
Genomic DNA is first captured by magnetic particles under optimized binding conditions. The remaining supernatant containing RNA is then transferred to a second adsorption step where RNA molecules bind to a separate magnetic particle system.
This staged adsorption design enables efficient recovery of both DNA and RNA from a single digestion process while maintaining compatibility with fragmented nucleic acids commonly present in FFPE tissues.
Engineering Characteristics
Magnetic Particle Adsorption
Silica-coated magnetic particles provide efficient adsorption of fragmented DNA and RNA commonly present in FFPE tissue lysates.
DNA/RNA Co-Isolation Workflow
The workflow enables recovery of both nucleic acid types from a single digestion process, supporting multi-omics analysis from limited FFPE tissue material
Fragment Recovery Performance
Magnetic bead binding conditions support recovery of short and degraded nucleic acid fragments typical of formalin-fixed tissues.
Low-Input Sample Recovery
Optimized bead-to-lysate ratios improve adsorption efficiency when processing low-concentration FFPE lysates.
Technical Validation
Recovery Across Sample Concentrations
Experimental evaluation using FFPE lysates with varying nucleic acid concentrations demonstrated consistent recovery across replicate extractions.
RNA Integrity and Stability
Electrophoresis analysis showed intact RNA bands with no detectable degradation, including samples stored overnight at 4 °C after extraction.
PCR Compatibility
RT-PCR experiments demonstrated comparable amplification efficiency between extracted RNA and reference viral controls, indicating absence of PCR inhibition.
Production Stability
Magnetic particle physicochemical parameters and extraction performance remained stable across validated production batches.
Kit Contents
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Contents
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R632701
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R632702
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R632703
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Purification Times
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48 Preps
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96 Preps
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5 x 96 Preps
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MagBind Particles
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1.1 ml
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2.5 ml
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11 ml
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MagPure Particles N
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1.1 ml
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2.5 ml
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11 ml
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Proteinase K
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24 mg
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48 mg
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220 mg
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Protease Dissolve Buffer
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3 ml
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10 ml
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15 ml
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Buffer DPS
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50 ml
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100 ml
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2 x 250 ml
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Buffer ATL
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20 ml
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30 ml
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120 ml
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Buffer BST1
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20 ml
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40 ml
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200 ml
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Buffer BXW1*
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44 ml
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110 ml
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3 x 110 ml
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RNase Free Water
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15 ml
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30 ml
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120 ml
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Storage and Stability
Proteinase K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stablefor at least 18 months under these conditions.
Experiment Data
