HiPure Universal DNA Kit

The HiPure Universal DNA Kit (D3018) is designed for purification of genomic DNA from a wide range of biological samples using silica membrane spin column technology. The optimized extraction chemistry supports efficient DNA recovery from complex sample matrices including blood, tissue, buffy coat, body fluids, swabs, dried blood spots and sperm samples.

The kit combines Proteinase K digestion with silica membrane adsorption to efficiently release genomic DNA from biological samples while removing proteins, lipids and other contaminants. The resulting DNA is suitable for routine molecular biology workflows such as PCR amplification, genotyping analysis and HLA typing studies.

Within the Magen DNA extraction portfolio, the HiPure Universal DNA Kit is positioned as a practical column-based solution for laboratories handling mixed sample types in routine workflows. Rather than being optimized for a single matrix, it provides a unified purification route across multiple common sample types, which is useful when workflow simplicity and broad sample compatibility are both required.

Workflow

Workflow Overview

The HiPure Universal DNA Kit uses a silica column-based workflow for purification of total DNA from routine sample types including tissue, blood, body fluids, cultured cells and swab-based materials. After sample-specific pretreatment, DNA is released by protease-assisted lysis, captured on the silica membrane under ethanol-containing binding conditions, and recovered through washing, drying and elution.

Sample Handling Logic

The main variation between sample types lies in the pretreatment and digestion stage. Tissue samples require cutting and complete digestion, cultured cells require pellet resuspension before lysis, while blood and other liquid samples can usually enter lysis more directly. Once the binding conditions are established, the downstream purification steps remain largely consistent.

Time and Workflow Characteristics

Under typical manual operation, the overall workflow for routine samples is usually completed within about 30–90 minutes, depending mainly on sample pretreatment and digestion requirements. For detailed sample-specific conditions, workflow guidance and estimated processing times, please refer to the Sample-Specific Workflow Note in the Download section.

Specifications

Technical Validation

Validation experiments using multiple biological sample types demonstrated stable genomic DNA purification with purity ratios typically A260/280 around 1.7–1.9, indicating high-quality genomic DNA suitable for downstream molecular analysis workflows.

Extraction experiments across different tissue samples showed reproducible DNA yields with variation between replicate extractions typically below 10%, demonstrating stable purification performance across multiple sample types.

Purified genomic DNA produced clear high-molecular-weight bands in agarose gel electrophoresis and supported successful PCR amplification of target gene fragments, confirming compatibility with common molecular biology applications such as PCR amplification and genotyping analysis.

Comparative testing against commonly used commercial genomic DNA purification systems showed comparable DNA purity and recovery performance, supporting reliable DNA preparation for routine laboratory workflows.

Typical Applications

Genomic DNA purified using the HiPure Universal DNA Kit is suitable for a wide range of molecular biology and molecular diagnostics research workflows, including:

• PCR amplification
• Genotyping analysis
• HLA typing studies
• Molecular diagnostics research
• Population genetics studies
• Sample preparation for sequencing workflows
• Biobanking sample processing

Related Products

For laboratories performing nucleic acid extraction from different biological samples or expanding molecular workflows, the following systems are commonly used together with the HiPure Universal DNA Kit.

• HiPure Blood DNA Mini Kit (D3111) – purification of genomic DNA from anticoagulated whole blood samples

• MagPure Universal DNA Kit (IVD3102) – magnetic bead-based purification of genomic DNA from mixed biological samples for automated extraction workflows

• HiPure Total RNA Plus Kit (R4111) – column-based purification of high-purity total RNA with genomic DNA removal

• MagPure Universal RNA Kit (IVD3020) – automated magnetic bead purification of RNA from tissue and cultured cells

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide