HiPure Circulating DNA Kit

How to read this note

1. Workflow structure
This workflow separates sample pretreatment from the shared downstream purification steps. It is intended as a practical companion to the product manual rather than a replacement for the official protocol. The downstream steps follow a silica membrane-based column purification workflow using a vacuum manifold system. This product also provides a centrifuge-based workflow option. For detailed procedures, refer to the HiPure Circulating DNA Spin Kit D (Cat. No. D3182D).

2. Time interpretation
Protocol times stated in the product manual are retained where applicable. Steps without explicit timing are estimated for an experienced operator. This workflow is continuous rather than incubation-dominated, but the 30 min lysis step remains the main fixed time block.

3. Workflow characteristics
This workflow is designed for large-volume plasma or serum input (1–5 mL) and emphasizes recovery of low-abundance fragmented DNA. Carrier RNA is included during lysis, followed by Buffer ACB conditioning on ice and vacuum-driven column loading for efficient handling of the full lysate volume.

4. Practical considerations
Use EDTA plasma where possible and avoid repeated freeze–thaw cycles. For routine 1 mL plasma input, refer to the HiPure Circulating Nucleic Acid Mini Kit (Cat. No. D3181). For limited or precious low-input samples around 0.6 mL, refer to the HiPure Circulating Nucleic Acid Micro Kit (Cat. No. D3180). For 1–5 mL plasma workflows where maximum fragmented DNA recovery is required, IVD3182 remains the core large-volume option.