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HiPure FFPE DNA/RNA Kit

FFPE DNA/RNA Split Workflow Note

Cat. No. IVD5116 · Representative split column workflow for parallel purification of DNA and RNA from the same FFPE input

Shared pretreatment Path-specific purification Partition / Elution

Workflow — Shared Pretreatment and Dual Purification Paths

DNA / RNA Partition

After the first lysis and centrifugation step, the RNA-containing supernatant and DNA-containing pellet are processed separately. This workflow therefore combines a shared FFPE pretreatment stage with two downstream column purification paths.

RNA Path

≈ 27.5 min

DNA Path

≈ 3 h 13 min

Estimated Total Time≈ 4.5 h

How to read this note

1. Workflow structure
This workflow separates shared FFPE pretreatment from the two downstream purification paths. It is intended as a practical companion to the product manual rather than a replacement for the official protocol. Both downstream paths follow silica membrane-based column purification, but they are entered after internal partitioning of the lysate.

2. Time interpretation
Protocol times stated in the product manual are retained where applicable. Steps without explicit timing are estimated for an experienced operator. For short protocol ranges, the timeline uses the midpoint. The total time reflects the shared pretreatment stage plus both downstream purification paths under the standard protocol, since both DNA and RNA are recovered from the same FFPE input.

3. Workflow characteristics
Unlike standard FFPE DNA-only or RNA-only workflows, this system separates RNA and DNA after the first lysis and centrifugation stage. RNA is purified from the supernatant after a shorter 80°C treatment, while the DNA-containing pellet is taken through a second lysis and a longer 56°C / 90°C sequence before column binding. This structural split is the defining feature of the workflow.

4. Practical considerations
The main technical risk in this workflow is not the downstream column processing itself, but the integrity of the partition step. Careful supernatant transfer is important to preserve the DNA pellet for the second path. In routine use, the RNA route is shorter and less heat-intensive, while the DNA route remains the dominant contributor to total workflow time because it includes an additional 56°C digestion step and a 90°C crosslink-reversal sequence.