HiPure FFPE RNA Kit

How to read this note

1. Workflow structure
This workflow separates sample pretreatment from the shared downstream purification steps. It is intended as a practical companion to the product manual rather than a replacement for the official protocol. The downstream steps follow a silica membrane-based column purification workflow.

2. Time interpretation
Protocol times stated in the product manual are retained where applicable. Steps without explicit timing are estimated for an experienced operator. This workflow separates active handling time from the shorter FFPE RNA incubation steps. For short protocol ranges, the timeline uses the midpoint. For long sample-dependent ranges, the timeline shows the shortest reasonable workflow path, while the broader range is explained in the note.

3. Workflow characteristics
Compared with FFPE DNA extraction, the defining upstream feature here is the shorter protease digestion and the 80°C incubation step used before column binding. This arrangement is intended to improve RNA accessibility while limiting excessive thermal exposure, since prolonged incubation may increase RNA degradation.

4. Practical considerations
RNA quality in FFPE workflows depends not only on extraction chemistry but also on fixation history, storage condition and operator handling. The core upstream goal is to achieve sufficient lysis without unnecessarily extending heat exposure. For DNA-sensitive downstream applications, the R4144 branch introduces an additional DNase treatment after lysis clarification and before column binding, rather than altering the downstream wash and elution structure.