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MagPure Circulating DNA Maxi Kit

5 mL Representative Plasma Workflow Note

Cat. No. IVD5435 · Representative magnetic-bead workflow for large-volume cfDNA extraction

Lysis and binding preparation Magnetic bead purification Elution

Workflow — 5 mL Representative Plasma Input (1–8 mL supported)

Estimated Total Time≈ 105–130 min

Input volume selection

Plasma Input	Binding Strategy	Typical Use Case
1–4 mL	Single-step binding	Routine magnetic cfDNA extraction
5–8 mL	Two-step binding	Low-abundance cfDNA or higher-sensitivity workflows

Reagent volumes should be adjusted according to sample input as specified in the product manual. This table is intended to help users select the appropriate handling logic rather than replace the detailed protocol tables.

How to read this note

1. Workflow structure
This workflow separates sample pretreatment from the shared downstream purification steps. It is intended as a practical companion to the product manual rather than a replacement for the official protocol. The downstream steps follow a magnetic bead-based purification workflow. This page uses a representative 5 mL plasma workflow, while the kit itself supports 1–8 mL plasma or serum input. For 1–4 mL inputs, the same workflow logic applies, but binding can usually be completed in a single loading step. For sample volumes above 4 mL, binding is performed in two sequential steps.

2. Time interpretation
Protocol times stated in the product manual are retained where applicable. Steps without explicit timing are estimated for an experienced operator. This workflow includes a 30–60 min incubation step for lysis, with additional handling time before and after incubation, followed by multi-step magnetic binding, washing, drying and elution procedures.

3. Workflow characteristics
This workflow is designed for large-volume cfDNA extraction using high-binding magnetic particles. Compared with column-based workflows, DNA capture is achieved through extended mixing with magnetic particles rather than membrane flow-through. Carrier RNA is optional but recommended, especially for low-abundance cfDNA samples, to reduce adsorption to consumables and stabilize yield. For sample volumes above 4 mL, lysate handling is performed in two sequential binding steps to maintain efficient particle-based recovery.

4. Practical considerations
For lower-volume magnetic cfDNA workflows (200–600 μL), refer to MagPure Circulating DNA Mini Kit (Cat. No. IVD5432). For vacuum-based column workflows from 1–5 mL plasma, refer to HiPure Circulating DNA Kit (Cat. No. IVD3182). For fragment-enrichment workflows designed to reduce larger DNA fragments and enrich shorter cfDNA fractions, refer to MagPure Circulating DNA Rich Mini Kit (Cat. No. 12917) and MagPure Circulating DNA Rich Maxi Kit (Cat. No. 12927). Use EDTA plasma where possible and avoid repeated freeze–thaw cycles before extraction.