MagPure FFPE DNA Kit (High Pure)
FFPE DNA Dual Binding Strategy Workflow Note
After FFPE lysis and lower-phase transfer, this workflow proceeds through one of two magnetic binding methods. High Salt Bind is used as the high-purity route, while Ethanol Bind is used as the high-yield route. These are alternative method options, not two sequential paths.
Method A: High Salt Bind
≈ 38 minMethod B: Ethanol Bind
≈ 38 minEthanol Bind ≈ 2.9 h
How to read this note
1. Workflow structure
This workflow separates shared FFPE sample pretreatment from two alternative downstream magnetic binding methods. It is intended as a practical companion to the product manual rather than a replacement for the official protocol. The downstream steps follow a magnetic bead-based DNA purification workflow after the chosen binding method is selected.
2. Time interpretation
Protocol times stated in the product manual are retained where applicable. Steps without explicit timing are estimated for an experienced operator. For short protocol ranges, the timeline uses the midpoint. Because High Salt Bind and Ethanol Bind are alternative method options, their times are shown separately rather than combined.
3. Workflow characteristics
D6323D differs from fragment-selection workflows by focusing on binding strategy rather than short-fragment removal. The High Salt Bind route uses BST1-driven binding conditions for high-purity DNA preparation, while the Ethanol Bind route introduces ethanol-mediated adsorption conditions for high-yield DNA preparation from FFPE lysate.
4. Practical considerations
Method selection should be made before the binding stage. The shared FFPE pretreatment remains the same, so differences between the two routes are concentrated in the binding setup rather than in deparaffinization, lysis or elution. Careful lower-phase transfer after FFPE pretreatment is still important, because the binding method cannot compensate for incomplete upstream recovery.