MagPure FFPE DNA/RNA Kit
FFPE DNA/RNA Sequential Adsorption Magnetic Workflow Note
In this workflow, DNA is first captured from the FFPE lysate by MagPure Particles N. The RNA-containing supernatant is then transferred to a separate magnetic binding route. This differs from early-partition workflows such as IVD3026, where RNA and DNA are separated before magnetic binding.
RNA Path
≈ 31 minDNA Path
≈ 25 minHow to read this note
1. Workflow structure
This workflow separates FFPE sample pretreatment from the sequential magnetic partition and the two downstream magnetic purification paths. It is intended as a practical companion to the product manual rather than a replacement for the official protocol. The downstream steps follow a magnetic bead-based purification workflow.
2. Time interpretation
Protocol times stated in the product manual are retained where applicable. Steps without explicit timing are estimated for an experienced operator. For short protocol ranges, the timeline uses the midpoint. The standard timeline shows the shortest reasonable workflow path, while the total range accounts for extended 56°C digestion when required by FFPE sample condition.
3. Workflow characteristics
R6327 uses a sequential adsorption strategy rather than an early lysis-stage split. DNA is captured first on magnetic particles from the FFPE lysate; the remaining supernatant is then used for RNA binding with a second magnetic particle system. This structure makes the initial DNA-binding step the central partition point of the workflow.
4. Practical considerations
The key handling point is accurate separation after the first magnetic binding step. The DNA-bound particles must be retained for the DNA path, while the RNA-containing supernatant should be transferred cleanly for RNA purification. Incompletely digested FFPE material can affect both paths, so digestion completeness upstream remains more important than modifying downstream wash steps.