MagPure Pathogen DNA/RNA Enrich Kit
Pathogen Enrichment and Magnetic Bead Purification Workflow
Key fraction logic
Plan B retains the viral / mycoplasma supernatant while the sediment undergoes host-background reduction and microbial disruption. Both fractions are then recombined before magnetic bead purification.
Plan B manual route; longer if optional repeated LBX1 treatment or vortex disruption is used.
How to read this note
1. Workflow structure
This workflow separates the Plan B pathogen enrichment module from the downstream manual magnetic bead purification path. It is intended as a practical companion to the product manual rather than a replacement for the official protocol. R6672C also includes a rapid Plan A direct extraction route and automated 32/48-channel and 96-channel extractor routes; this note focuses on Plan B because it best represents the host-background reduction and pathogen enrichment workflow.
2. Time interpretation
Protocol times stated in the product manual are retained where applicable. Steps without explicit timing are estimated for an experienced operator, including pipetting, tube transfer, centrifuge or magnetic-rack handling, supernatant / filtrate removal and tube or column repositioning. For short protocol ranges, the timeline uses the midpoint. For long or optional protocol ranges, the displayed standard timeline uses the shortest reasonable path, while the note and total-time range indicate where extended handling may apply. Cumulative time runs continuously from the first step to final elution across all workflow sections.
3. Workflow characteristics
R6672C uses an enrichment-before-purification strategy rather than simple direct lysis. The retained supernatant carries the viral / mycoplasma fraction, while the sediment is processed to reduce eukaryotic-cell background and release microbial nucleic acids through DNase treatment and bead-tube disruption. The retained supernatant is then combined with the microbial lysate as the final step of the enrichment module; Proteinase K conditioning and MPN9 magnetic particle binding mark the start of the shared magnetic bead method.
4. Practical considerations
The key handling point is clean retention of the supernatant before sediment processing. The retained supernatant should not be discarded. Cell sediment should be fully resuspended before LBX1 treatment, and excessive sediment may require an additional LBX1 wash / centrifugation cycle before DNase treatment. Efficient bead-tube disruption is especially important for bacterial, fungal and mixed microbial pathogen material.