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Your present location:Home/Products/DNA&RNA Purification/RNA/Tissue/Cell RNA/Column Kits/HiPure Total RNA Kit
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HiPure Total RNA Kit

IVD4121
CAT NO PRODUCT NAME SIZE PRICE
IVD4121 HiPure Total RNA Kit 50 preps $348.00

Introduction

This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA(miRNA)from tissue, cell using two columns and DNase plus reagent
Applications RT-PCR, cDNA synthesis, second generation sequencing
Products
RNA, miRNA
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Clinical tissues, cells, lymphocytes
Sample amount

Tissue: <20 mg

Cells: <5 x 106

Yield 2-50μg
Elution volume 30μl
Time per run ≤25 minutes


Principle

This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.


Advantages

  • Efficient DNA removal - one step RNA extraction can effectively remove genomic DNA
  • High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
  • Fast - the whole extraction only takes 15-25 minutes
  • Nontoxic - no toxic phenol chloroform extraction is required in the extraction


Kit Contents

Contents IVD4121
Purification Times 50 Preps
HiPure DNA Mini Column Ⅱ
50
HiPure RNA Mini Columns 50
2ml Collection Tubes 150
Proteinase K
24 mg
Protease Dissolve Buffer
1.8 ml
DNase I
600 μl
DNase Buffer
6 ml
Buffer RTL
40 ml
RNA Digestion Buffer
15 ml
Buffer RWC* 20 ml
Buffer RW2*
20 ml
Nuclease Free Water
10 ml


Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

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