| CAT NO | PRODUCT NAME | SIZE | PRICE |
| IVD4173 | HiPure Viral RNA/DNA Kit | 100 preps | $249.00 |
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Specifications
| Features | Specifications |
| Main Functions | Extract viral RNA/DNA from 200μl plasma/serum samples |
| Applications |
RT-PCR，PCR，NGS |
| Products |
Viral total nucleic acid, body cell total nucleic acid, negative bacterial DNA |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type |
Whole blood, plasma, serum, soaking solution and tissue homogenate supernatant |
| Sample amount | 200μl |
| Yield |
2-10μg |
| Elution volume | ≥30μl |
| Time per run |
≤30 minutes |
| Liquid carrying volume per column |
800μl |
| Binding yield of column | 100μg |
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Kit Contents
| Contents | IVD4173 |
| Purification Times | 100 Preps |
| HiPure Viral Mini Column | 100 |
|
2ml Collection Tubes |
200 |
| PK/Carrier RNA |
50 mg |
| Protease Dissolve Buffer | 5 ml |
|
Buffer AL |
30 ml |
|
Buffer MW1 |
44 ml |
|
Buffer MW2 |
50 ml |
|
RNase Free Water |
15 ml |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
Experiment Data
D3018
IVD5412
IVD4173
IVD3020
R4011
D6364
D3141
D3146
D6351
D3187
D3125
D3126
IVD5432
D3182C
IVD3102
D3111
