| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R214402 | HiPure RNA Clean Up Kit | 50 preps | $120.00 |
| R214403 | HiPure RNA Clean Up Kit | 250 preps | $430.00 |
Specifications
| Features | Specifications |
| Main Functions | Recovery of single stranded or double stranded RNA from crude RNA products and enzymatic reaction solution |
| Applications |
Sequencing, ligation, enzyme digestion, RT-PCR, labeling, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type |
Crude RNA, enzymatic reaction solution |
| Sample amount | ≤100μl |
|
Recovery |
90% |
| Elution volume | ≥15μl |
| Time per run |
≤15 minutes |
| Liquid carrying volume per column |
800μl |
| Binding yield of column | 100μg |
The HiPure system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the sample or other enzymatic reaction, and the mixture is applied to the column. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure RNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.
Advantages
Kit Contents
| Contents | R214402 | R214403 |
| Purification Times | 50 Preps |
250 Preps |
| Buffer GXP | 30 ml | 120 ml |
|
Buffer RW2 |
20 ml |
2 x 50 ml |
| RNase-Free Water |
20 ml |
60 ml |
| HiPure RNA Mini Columns I | 50 |
250 |
|
2 ml Collection Tubes |
50 |
250 |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
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IVD4173
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D3146
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D3126
IVD5432
D3182C
IVD3102
D3111
