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HiPure Viral DNA/RNA Kit

IVD4175
CAT NO PRODUCT NAME SIZE PRICE
IVD4175 HiPure Viral RNA Kit 100 preps $249.00

Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details


Specifications

Features Specifications
Main Functions Rapidly Extract viral DNA/RNA from 200μl non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, etc.
Applications RT-PCR,PCRNGS
Products Viral DNA / RNA, body cell DNA / RNA
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Plasma, serum, effusion, urine, fecal suspension supernatant
Sample amount 200μl
Elution volume 30μl
Time per run
Liquid carrying volume per column 800μl
Binding yield of column 100μg


Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).


Advantages

  • Fast - column purification without PK digestion
  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Safe - no phenol chloroform extraction required
  • Sensitive - DNA/RNA can be recovered at the level of PG


Kit Contents

Contents IVD4175
Purification Times 100 Preps
HiPure Viral Column 100
2ml Collection Tubes
100
PK/Carrier RNA 50 mg/310μg
Protease Dissolve Buffer 5 ml
Buffer VLE
42 ml
Buffer CE
60 ml
RNase Free Water
15 ml


Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

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