HiPure Plasmid Mini Kit II is thelarger-input support configuration within the standard Magen plasmid mini-prepworkflow. It retains the same alkaline lysis and silica membrane logic asP1001, but is positioned for laboratories that routinely process 5–15 mLovernight culture and want to stay in a familiar single-column manual format.

For routine smaller-input plasmidpreparation, HiPure Plasmid Mini Kit (P1001) remains the standard core product.For users moving upward in preparation scale, HiPure FastFilter Plasmid MidiKit (P1013) provides the next column-based step. Laboratories requiringlow-endotoxin DNA should move to the EF line rather than the standard mini-prepline.

Specifications

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast - it takes only 2 minutes to obtain supernatant by optimized solution (10 minutes for other brands)
• High yield - up to 70µg plasmid can be binded in one column

Kit Contents

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

For guidance on selecting between standard and low-endotoxin plasmid workflows based on copy number, culture input and processing format, please refer to the Plasmid DNA Kits Purchase Guide.