Magnetic Bead Plasmid DNA Extraction Kit | High-Throughput Mini Prep

Introduction

MagPure Plasmid Mini Kit represents the standard magnetic bead branch within the Magen plasmid system. Rather than extending the manual column workflow, it provides a separate route built for high-throughput handling, deep-well plate processing and automation-compatible plasmid purification.

For low-endotoxin magnetic preparation, the corresponding support product is MagPure Plasmid EF Mini Kit(P1814). For standard column workflows, users should remain within the HiPure Plasmid Mini Kit(P1001) / P1002 / P1006 branch.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 20µg plasmid DNA from 1-3ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Conventional plasmid, plasmid≤30KB
Sample amount	1-3ml
Elution volume	≥50μl

Engineering Characteristics

High-throughput magnetic workflow

The system is designed for deep-well plate handling and magnetic separation,making it more suitable for batch processing and automation-oriented laboratories than for routine single-column mini-prep use.

PW1-supported RNA control

Internal testing showed that bead-to-lysate ratio affects apparent plasmid yield. A PW1 wash step is recommended when reduced RNA interference and morereliable OD-based quantification are important, especially for medium- tolow-copy plasmids.

Batch-friendly and automation-oriented

The workflow is structured around repeated pipetting, shaking and magnetic separation steps, which makes it easier to integrate into automated or semi-automated laboratory formats.

Technical Validation

RNA carryover depends on wash conditions

Internal testing showed that increasing bead input without PW1 washing could raise OD-based yield partly through RNA carryover rather than true plasmid gain.

PW1 improves preparation quality

With PW1 washing, plasmid recovery became more consistent and better aligned with routine preparation expectations, particularly for medium- to low-copy plasmids.

High-throughput usability

Plate-based testing across multiple bacterial samples supported the product's role in batch and automation-oriented workflows.

Kit Contents

Contents	P181102	P181103	P181104
Purification Times	100 Preps	500 Preps	5000 Preps
RNase A	10 mg	50 mg	2 x 250 mg
Buffer P1	30 ml	150 ml	2 x 750 ml
Buffer P2	30 ml	150 ml	2 x 750 ml
Buffer N3	30 ml	150 ml	2 x 750 ml
Buffer PW1	35 ml	180 ml	2 x 900 ml
MagPure Particle NB*	2.2 ml	11 ml	2 x 60 ml

Storage and Stability

RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.

Experiment Data

Purchase Guide

For guidance on selecting between standard and low-endotoxin plasmid workflows based on copy number, culture input and processing format, please refer to the Plasmid DNA Kits Purchase Guide.

Articles

【Nature Catalysis】Efficient exploration of terpenoid biosynthetic gene clusters in filamentous fungi

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P1811 MagPure Plasmid Mini Kit 2022.pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
P181102	MagPure Plasmid Mini Kit	100 preps	$59.00
P181103	MagPure Plasmid Mini Kit	500 preps	$239.00
P181104	MagPure Plasmid Mini Kit	5000 preps	$2,152.00

MagPure Plasmid Mini Kit

Introduction

Details

Specifications

Engineering Characteristics

High-throughput magnetic workflow

PW1-supported RNA control

Batch-friendly and automation-oriented

Technical Validation

RNA carryover depends on wash conditions

PW1 improves preparation quality

High-throughput usability

Kit Contents

Storage and Stability

Experiment Data

Purchase Guide

Articles

Download

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MagPure Plasmid EF Mini Kit

HiPure Plasmid Mini Kit

HiPure Plasmid Mini Kit II

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