Plasmid DNA purification should be selected according to downstream purity requirements, plasmid copy number, culture input, expected output and processing format. A routine cloning workflow does not require the same purification strategy as cell transfection, animal injection, concentrated plasmid preparation or milligram-scale production.
Magen provides standard molecular biology-grade, low-endotoxin and endotoxin-free plasmid preparation systems based on spin columns, filter-assisted columns, 96-well plates and magnetic bead platforms. The portfolio is organized around six Core products. Support products extend each Core route by culture scale, plasmid copy number, target yield or instrument configuration.
| Purity Grade | Endotoxin Level | Typical Applications | Selection Direction |
| Standard molecular biology grade | Not specified as an EF workflow | Cloning, PCR, restriction digestion and routine sequencing | P1001 or P1811 branches |
| Low Endotoxin | <1 EU/µg | Cell transfection and endotoxin-sensitive in vitro workflows | Choose by copy number, required output and manual or automated format |
| Endotoxin Free | <0.1 EU/µg | Animal injection and other injection-grade plasmid workflows | Select products with a dedicated or Additional Protocol for endotoxin-free preparation |
For medium- to low-copy plasmids, large constructs or workflows requiring reduced RNA interference, start with a high-salt-mediated route. Alcohol-mediated binding provides broad recovery and is suitable for routine or high-copy plasmids when total recovery is the main priority. Cation-mediated selective precipitation is intended for high-copy plasmids requiring very high total yield or concentrated recovery.
High total yield and high final concentration are related but are not the same selection target. P1116 and its supporting formats are designed for high total output from high-copy plasmids. P1231 and its related products emphasize concentrated recovery through selective enrichment and smaller-volume elution.
Use spin-column or filter-assisted systems for flexible manual processing, 96-well column plates for parallel preparation, liquid-handling configurations for programmable reagent transfer, and magnetic bead systems for automation-oriented batch processing. Instrument compatibility should be considered together with required throughput and whether mid-run reagent addition is acceptable.
| Route | Best Suited For | Main Strength | Selection Consideration | Representative Routes |
| Alcohol-mediated | Routine and high-copy plasmids | Broad plasmid recovery and high total output | RNA may be co-recovered, particularly when plasmid abundance is low; OD concentration should be interpreted with electrophoresis when necessary | P1001, P1156B and P1811 branches |
| High-salt-mediated | Medium-, low- and ultra-low-copy plasmids | Reduced RNA interference and more representative OD-based plasmid quantification | A260/A230 may be lower under some low-yield conditions; the route prioritizes selectivity rather than maximum apparent OD yield | P1154, P1157, P1230, P1814 and P1816 |
| Cation-mediated | High-copy plasmids | Selective plasmid enrichment for high total yield or concentrated recovery without relying solely on fixed membrane capacity | Not the preferred route for medium- or low-copy large constructs; confirm expected plasmid yield per millilitre before selection | P1116, P1231 and P1815 branches |
| Product | Format | Purity Grade | Input & Yield | Plasmid Type | Selection Point |
|
HiPure Plasmid Mini Kit (P1001) CORE |
Mini spin column | Standard |
1–5 mL Up to 35 µg |
General plasmids | Routine manual plasmid preparation for cloning, PCR, digestion and sequencing |
|
HiPure Plasmid Mini Kit II (P1002) |
Larger-input mini column | Standard |
5–15 mL Up to 70 µg |
General plasmids | More culture input while remaining within a routine mini-prep format |
|
HiPure Plasmid Plus 96 Kit (P1006) |
96-well column plate | Standard |
1.5 mL/well Up to 20 µg |
General plasmids | Parallel screening and batch preparation without magnetic handling |
|
HiPure FastFilter Plasmid Midi Kit (P1013) |
Midi column + FastFilter | Standard |
25–50 mL Up to 250 µg |
General plasmids | Mid-scale preparation with rapid filter-assisted lysate clarification |
|
HiPure FastFilter Plasmid Maxi Kit (P1014) |
Maxi column + FastFilter | Standard |
100–200 mL Up to 1 mg |
General plasmids | Large-scale standard preparation with faster clarification |
| Product | Format | Purity Grade | Input & Yield | Plasmid Type | Selection Point |
|
HiPure Plasmid EF Maxi Kit (P1156) CORE |
Maxi spin column |
Low Endotoxin; Endotoxin Free via Additional Protocol |
100–200 mL 100–1,500 µg |
High-, medium- and low-copy plasmids | Broad routine EF compatibility; transfection and animal injection routes within one product |
|
HiPure Plasmid EF Mini Kit (P1154) |
Mini spin column | Low Endotoxin |
5–15 mL Up to 70 µg |
Particularly medium- to low-copy plasmids | High-salt mini workflow with reduced RNA interference for transfection |
|
HiPure Plasmid EF 96 Kit (P1157) |
96-well column plate | Low Endotoxin |
1.3–1.5 mL/well Up to 20 µg |
General, especially medium- to low-copy plasmids | Parallel low-endotoxin preparation with high-salt RNA control |
|
HiPure Plasmid EF Mega Kit (P1159) |
Large-format column | Endotoxin Free |
200–500 mL Up to 2,000 µg |
High-, medium- and low-copy plasmids | Extended-scale injection-grade preparation with broad vector compatibility |
| Product | Format | Purity Grade | Input & Yield | Plasmid Type | Selection Point |
|
HiPure HY Plasmid EF Mega Kit (P1116) CORE |
Mega filter-column workflow |
Low Endotoxin; Endotoxin Free via Additional Protocol |
500 mL Up to 5 mg |
High-copy plasmids | Core cation-mediated route for milligram-scale plasmid production |
|
HiPure HY Plasmid EF 96 Kit (P1115) |
96-well filter plate | Low Endotoxin |
1–5 mL/well Up to 50 µg |
High-copy plasmids producing ≥3 µg/mL | High-yield 96-well preparation for transfection; not intended for low-copy large constructs |
|
HiPure HY Plasmid EF Giga Kit (P1117) |
Giga filter-column workflow |
Low Endotoxin; Endotoxin Free via Additional Protocol |
1–1.5 L Up to 10 mg |
High-copy plasmids | Production scale above P1116 while retaining the same high-yield logic |
|
HiPure HY Plasmid EF 10L Kit (P1118) |
Multi-litre production workflow |
Low Endotoxin; Endotoxin Free via Additional Protocol |
10–15 L Up to 100 mg |
High-copy plasmids | Highest-output configuration for multi-litre plasmid production |
| Product | Format | Purity Grade | Input & Yield | Plasmid Type | Selection Point |
|
HiPure Plasmid EF Midi Plus Kit (P1231) CORE |
Compact column + extender |
Low Endotoxin; Endotoxin Free via Additional Protocol |
25–50 mL Up to 500 µg |
High-copy plasmids | Cation-mediated selective enrichment with small-volume elution for concentrated recovery |
|
HiPure Low Copy Plasmid EF Kit (P1230) |
High-salt compact column | Low Endotoxin |
20–100 mL Up to 100 µg |
Low- and ultra-low-copy plasmids, including low-copy lentiviral constructs | Increased culture input and high-salt selectivity for low-abundance vectors |
|
HiPure Plasmid EF Maxi Plus Kit (P1233) |
Maxi selective-enrichment column |
Low Endotoxin; Endotoxin Free via Additional Protocol |
100–200 mL Up to 1.5 mg |
General plasmids | Larger-scale concentrated plasmid recovery with approximately 300 µL elution |
| Product | Format | Purity Grade | Input & Yield | Plasmid Type | Selection Point |
|
MagPure Plasmid Mini Kit (P1811) CORE |
96-well magnetic bead workflow | Standard |
1.3–1.7 mL/well Up to 20 µg |
General plasmids | Core alcohol-mediated magnetic workflow for high-throughput batch preparation |
|
MagPure Fast Plasmid Mini LQ Kit (P1812) |
Liquid-handling workstation | Standard |
1–3 mL Up to 20 µg |
General plasmids | Magnetic impurity removal plus alcohol-mediated binding for fast, cost-controlled liquid handling |
|
MagPure Fast Plasmid Mini KF Kit (P1813) |
Prefilled 96-channel magnetic workflow | Standard |
1–3 mL Up to 20 µg |
General plasmids | Continuous instrument operation without mid-run reagent addition after plate loading |
| Product | Format | Purity Grade | Input & Yield | Plasmid Type | Selection Point |
|
MagPure Plasmid EF Mini Kit (P1814) CORE |
Manual or automated magnetic workflow | Low Endotoxin |
1–5 mL Up to 15 µg |
Medium- to low-copy plasmids | Core high-salt magnetic route with reduced RNA interference for cell transfection |
|
MagPure Plasmid EF Midi Kit (High Copy) (P1815) |
Magnetic midi workflow; M8/M24 configurations | Low Endotoxin |
Standard: 50–75 mL / up to 500 µg M8: 50–80 mL / up to 500 µg M24: 100–200 mL / up to 2,000 µg |
High-copy plasmids producing >5 µg/mL | Cation-mediated high-output magnetic route for cell transfection |
|
MagPure Plasmid EF Midi Kit (Medium/Low Copy) (P1816) |
Magnetic midi workflow; M8/M24 configurations | Low Endotoxin |
Standard/M8: 50–75 mL / up to 250 µg M24: 100–200 mL / up to 1,000 µg |
Medium- to low-copy plasmids | High-salt magnetic route for improved RNA control in cell-transfection workflows |
Plasmid copy number is determined primarily by the vector replication origin and its regulatory elements, but actual plasmid output is also affected by the host strain, insert size and sequence, antibiotic selection, culture medium and growth conditions. Vector names should therefore be treated as practical references rather than absolute copy-number classifications.
| Plasmid Context | Typical Examples | Selection Implication |
| Typically higher-copy | pUC-derived, pBluescript-type and pGEM-type cloning plasmids; many small routine cloning vectors | Useful yield may be reached from lower culture input. High-yield or high-concentration routes can be selected when total output is the priority. |
| Typically lower-copy or variable-output | BACs, fosmids, cosmids, plasmids above 10 kb, lentiviral constructs and other large functional vectors | Higher culture input and high-salt selective purification may be more appropriate. Avoid assuming that a high-yield high-copy route will improve recovery. |
Final selection note: stated culture input and yield represent recommended workflow ranges rather than guaranteed output for every vector. Plasmid copy number, insert characteristics, bacterial growth and purification capacity should be considered together. For detailed operating conditions and configuration-specific instructions, refer to the corresponding product page and protocol.
