Magnetic bead–based nucleic acid extractionrelies on the selective binding of DNA or RNA to functionalized particlesurfaces under specific buffer conditions. Silica-coated beads typically bindnucleic acids in the presence of chaotropic salts, while impurities such asproteins and inhibitors remain in solution.
This solid-phase extraction mechanism enables rapid separation, washing andelution steps within a single tube, supporting high-throughput workflows andautomation compatibility. Compared with traditional methods, magnetic beadsystems simplify handling steps and improve reproducibility across differentsample types.
Different magnetic bead formulations vary in surface chemistry, particle sizeand binding behavior, making them suitable for specific applications such aslow-input samples, circulating DNA or complex biological matrices.
Magnetic bead selection depends on sample input, nucleic acid abundance and fragment characteristics. Different bead types are optimized for specific workflow conditions rather than serving as universal solutions.
Recommended:
Provide stable DNA yield across tissue and blood samples, with comparable purity and reproducibility.
Also suitable for DNA/RNA clean-up workflows integrated within extraction systems.
Recommended:
Optimized for nucleic acid recovery in low-input and complex biological samples.
Demonstrated higher nucleic acid yield in low-in put and complex biological samples compared to standard silica beads
Recommended:
Designed for efficient recovery of short DNA fragments in large-volume plasma workflows.
Shows consistently higher recovery of short DNA fragments and improved yield in plasma samples compared to other bead types
Recommended:
Suitable for fragmented DNA workflows, with recovery of very short fragments dependent on binding conditions.
Recommended:
PEG-mediated binding system designed for nucleic acid clean-up and size selection in enzymatic and assay workflows.
It is also used in selected Magen FFPE DNA/RNA co-extraction workflows, particularly in dual-bead processing configurations.
| Features | MagPure Particles |
MagPure Particles N |
MagPure Particles G |
MagPure Particles F |
MagBind Particles |
|
Cat.No. |
C1410 | C1411 | C1414 |
C1413 |
|
|
Concentration |
100mg/ml | 70mg/ml |
40mg/ml |
50mg/ml |
10mg/ml |
|
Form |
Amorphous and Porous | Amorphous and Porous |
Porous |
Amorphous |
Nonporous |
|
Surface function |
Si-OH, Silica Beads | Si-OH, Silica Beads |
Si-OH, Silica Beads |
Si-OH, Silica Beads |
COOH, Carboxyl Beads |
|
Dispersion |
Polydisperse | Polydisperse |
Monodisperse |
Monodisperse |
Monodisperse |
|
Particle Size |
1.5-5μm | 0.2-2μm |
1-1.5μm |
0.2-1.5μm |
0.8-1μm |
|
Color |
Black | Yellowish Brown |
Dark Brown |
Dark Brown |
Yellowish Brown |
|
Magnetic response |
15-30s | ~60s |
~30s |
20s |
120s |
|
Settling Time (1ml) |
>5min | >10min |
>3min |
>3min |
>2h |
|
Usage (0.2ml Sample) |
20μl | 20μl |
20-30μl |
20-30μl |
20-30μl |
|
DNA Recover Rate (only 4M GITC) |
>80% |
>80% |
>80% |
>80% | Not applicable (PEG-based system) |
|
DNA Recover Rate (10% PEG8000/NaCl) |
>85% |
>85% |
>85% |
>85% |
>90% |
|
Core feature |
Universal silica magnetic beads |
Small-size high surface area beads |
Fragment DNA optimized beads |
Large-volume cfDNA optimized beads |
Carboxyl-coated beads |
|
Best For (Typical Applications) |
|
|
|
|
|
|
Magen kits |
IVD3102 |
D6364, IVD5412 |
IVD5432, 12927 |
IVD5435 |
IVD3101, IVD3026, R6327 |
