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Your present location:Home/Products/Raw Materials and Consumables/Magnetic Bead/DNA/RNA binding bead/MagPure Particles
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MagPure Particles

C1410
CAT NO PRODUCT NAME SIZE PRICE
C14100 MagPure Particles 100 ml $599.00
C14101 MagPure Particles 400 ml $2,199.00
C14102
MagPure Particles
3 x 400 ml
$5,999.00
C14103
MagPure Particles
10 x 400 ml
$15,999.00

Introduction

MagPure Particles represent the standard silica-based magnetic bead system within the Magen sample preparation portfolio. The particles are designed for routine DNA and RNA purification from biological samples including tissue, blood and cultured cells.
The system provides consistent adsorption behavior under chaotropic binding conditions and is compatible with a wide range of extraction buffers and workflows.

Details

Specifications

Features Specifications
Concentration 100 mg/ml
Appearance Suspension of black particles
Surface functional group Si-OH, Silanol
Dispersibility Polydisperse Amorphous
Particle size 1.5-5 μm
Preservation conditions

Room Temperature, valid for up to 2 years.

It is recommended to store in 2-8°C to prevent microbial growth.

Magnetic response speed
15-30 seconds
Settling velocity
>5 minutes
High salt mediated binding
>2M guanidine isothiocyanate, DNA recovery up to 80%
Alcohol mediated binding
2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85%
PEG8000 mediated binding
The recovery of DNA/RNA was up to 85%
DNase/RNase
Not detected
DNA residue
<1 ppm
Recommended application
Plasmid extraction, gel DNA recovery, genomic DNA extraction and RNA extraction.


Principle

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.

After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.


Ordering information

CAT.No. Product Name
Package
C14100
MagPure Particles
100 ml
C14101
MagPure Particles
400 ml
C14102
MagPure Particles
3 x 400 ml
C14103
MagPure Particles
10 x 400 ml

Purchase Guide

Related Bead Selection

For full comparison across all magnetic bead systems, refer to the Magnetic Bead Selection Guide.

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