Introduction
The MagPure Universal DNA Kit (IVD3102) is designed for automated purification of genomic DNA from diverse biological samples using magnetic bead purification technology. The extraction chemistry enables efficient DNA recovery from multiple sample types including whole blood, buffy coat, cultured cells, tissue homogenates and other biological materials commonly processed in molecular laboratories.
Magnetic bead purification allows selective binding of genomic DNA molecules while enabling automated washing and elution steps through magnetic separation. Compared with manual column workflows, magnetic bead extraction systems provide improved workflow consistency and support high-throughput nucleic acid purification.
The MagPure Universal DNA Kit is compatible with automated nucleic acid purification instruments and supports routine DNA extraction workflows in research laboratories, clinical molecular laboratories and high-throughput testing environments.
Within the Magen DNA extraction systems, the MagPure Universal DNA Kit represents the magnetic bead-based universal DNA extraction platform, complementing the column-based HiPure Universal DNA Kit (D3018) for laboratories requiring automated DNA purification workflows.
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation total DNA from blood, buffy coat, tissue and other samples
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Applications
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Second generation sequencing, PCR, real time PCR, etc.
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Purification method
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Polydisperse magnetic beads
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Sample type
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Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
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Sample amount
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Whole blood :< 200μl; Saliva / swab:< 400μl; Tissue :< 20mg
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Yield
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0.1 - 50μg
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Elution volume
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Time per run
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Engineering Characteristics
Magnetic bead DNA adsorption technology
The purification workflow uses high-affinity magnetic particles that selectively bind genomic DNA under optimized buffer conditions. Magnetic separation allows efficient removal of proteins, cellular debris and inhibitors during washing steps.
Broad sample compatibility
The extraction chemistry supports purification of genomic DNA from multiple biological sample types including blood, cultured cells, tissue samples and other biological matrices frequently processed in molecular biology laboratories.
Automation-ready workflow
The kit is designed for compatibility with automated nucleic acid purification systems, supporting multi-channel extraction workflows for routine laboratory processing.
Consistent purification performance
Magnetic bead-based purification provides stable DNA recovery and reduces manual handling variability compared with manual extraction procedures.
Flexible laboratory integration
The extraction workflow can be integrated into automated sample preparation pipelines commonly used in high-throughput laboratories.
Technical Validation
Validation experiments demonstrated stable genomic DNA recovery across multiple biological sample types processed using magnetic bead purification workflows. Purified DNA showed purity ratios consistent with high-quality genomic DNA suitable for PCR amplification and downstream molecular analysis.
The magnetic bead extraction workflow enables efficient removal of inhibitors originating from biological samples, supporting reliable performance in molecular biology workflows including PCR amplification and genotyping analysis.
Comparative evaluations with commonly used genomic DNA purification systems demonstrated comparable DNA purity and recovery, supporting routine use in automated nucleic acid extraction workflows.
Typical Applications
Genomic DNA purified using the MagPure Universal DNA Kit is suitable for a wide range of molecular biology and molecular diagnostics research workflows, including:
• PCR amplification
• Genotyping analysis
• HLA typing studies
• Molecular diagnostics research
• Sample preparation for sequencing workflows
• Population genetics studies
• High-throughput nucleic acid extraction laboratories
Related Products
Laboratories performing nucleic acid extraction from diverse biological samples often combine automated DNA purification with additional DNA and RNA extraction systems.
• HiPure Universal DNA Kit (D3018) – column-based purification of genomic DNA from mixed biological samples including blood, tissue, swabs and body fluids
• HiPure Blood DNA Mini Kit (D3111) – purification of genomic DNA from whole blood samples using silica membrane spin columns
• MagPure Forensic DNA Kit (D6359D) – magnetic bead purification of trace DNA from forensic samples including hair roots, blood stains and bone fragments
• MagPure Universal RNA Kit (IVD3020) – automated purification of total RNA from tissue and cultured cells
• HiPure Total RNA Plus Kit (R4111) – column-based purification of high-purity total RNA with genomic DNA removal
Kit Contents
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Contents
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IVD3102
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Purification Times
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200
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MagPure Particles
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5 ml
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Proteinase K
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100 mg
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Protease Dissolve Buffer
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10 ml
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Rnase A
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40 mg
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Buffer ATL
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60 ml
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Buffer AL
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60 ml
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Buffer BD*
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20 ml
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Buffer BW1*
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110 ml
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Elution Buffer
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30 ml
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Cat.No
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Reagent
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IVD3102-F-96
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Proteinase K
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50 mg
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Protease Dissolve Buffer
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6 ml
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RNase A
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20 mg
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Buffer ATL
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30 ml
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Buffer AL
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30 ml
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96-Tip
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1
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Sample plate (DW Plate)
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450µl Buffer BD(Ethanol Added)
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1
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Wash 1 Plate (DW Plate)
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600µl Buffer BW1(Ethanol Added)
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1
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Wash 2 Plate (DW Plate)
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600µl Buffer BW1(Ethanol Added)
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1
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Wash 3 Plate (DW Plate)
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750µl Buffer GW2, 20µl MagPure Particle
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1
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Elution plate (DW Plate)
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80µl Elution Buffer
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1
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Cat.No
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Reagent
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IVD3102-TL-06
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Proteinase K
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50 mg
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RNase A
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20 mg
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Protease Dissolve Buffer
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6 ml
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Buffer ATL
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40 ml
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Buffer AL
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40 ml
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AS-Tip
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12
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2.0ml V-bottom plate
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Row 1/7：450µl Buffer BD
Row 2/8：450µl Buffer BW1
Row 3/9：450µl Buffer BW1
Row 4/10：20μl Magpure Particle
450μl Wash Buffer GW2
Row 5/11：450μl Wash Buffer GW2
Row 6/12：80µl Elution Buffer
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6
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Storage and Stability
Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
