Introduction
The HiPure Tissue DNA Kit is designed for routine purification of genomic DNA from animal tissues and cultured cells using silica membrane spin column technology. The workflow supports rapid lysis and efficient removal of proteins and enzymatic inhibitors, allowing recovery of high-quality DNA suitable for PCR amplification, sequencing preparation, and genotyping studies.
The system is optimized for processing up to 25 mg tissue or approximately 5 × 10⁶ cultured cells per preparation, making it suitable for routine molecular biology laboratories working with standard tissue samples and cell culture models.
Within the Magen Blood & Tissue DNA extraction portfolio, this kit serves as a tissue-focused workflow. Laboratories processing diverse biological samples may alternatively use the HiPure Universal DNA Kit (D3018) or the automated MagPure Universal DNA Kit (IVD3102).
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation total DNA from <25 mg tissue, culture cells, FTA card
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Applications
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PCR, southern bolt and virus detection, etc.
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Purification method
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Mini spin column
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Purification technology
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Silica technology
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Process method
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Manual (centrifugation or vacuum)
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Sample type
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Animal tissue or cultured cells
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Sample amount
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Animal tissue : <25mg, Cultured cells : <5 x 106
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Elution volume
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≥30μl
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Time per run
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30 - 60 minutes
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Liquid carrying volume per column
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800μl
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Binding yield of column
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100μg
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Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins andother impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).
Technical Validation
The HiPure Tissue DNA Kit was validated using multiple animal tissue samples, including mouse ear, mouse tail, chicken kidney, chicken brain, frog spleen, frog skin, frog lung, fish liver, fish heart and fish muscle. Across the tested tissue types, purified DNA showed A260/280 values mainly within 1.70–1.90, with replicate extractions showing stable recovery and yield variation generally within 10%.
Agarose gel electrophoresis showed clear high-molecular-weight genomic DNA bands, indicating good DNA integrity. Purified DNA was further tested by HindIII restriction digestion and PCR amplification of a 1000 bp β-actin fragment, supporting compatibility with enzymatic downstream applications and routine PCR analysis.
Additional validation using 20 mg shrimp muscle tissue showed DNA concentrations of approximately 72.5–81.8 ng/µL and yields of approximately 3.6–4.1 µg with a 50 µL elution volume. Gel electrophoresis showed clear DNA bands without obvious degradation, supporting reliable DNA extraction from muscle-type animal tissue samples.
Kit Contents
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Contents
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D312102
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D312103
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Purification Times
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50 Preps
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250 Preps
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Buffer ATL
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15 ml
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65 ml
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Buffer DL
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15 ml
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65 ml
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Buffer GW1
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22 ml
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110 ml
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Buffer GW2
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12 ml
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50 ml
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RNase A
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10 mg
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50 mg
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Proteinase K
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24 mg
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120 mg
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Protease Dissolve Buffer
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5 ml
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15 ml
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Buffer AE
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15 ml
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60 ml
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HiPure gDNA Mini Columns
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50
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2 x 125
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2 ml Collection Tubes
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100
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5 x 100
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Storage and Stability
RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
