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Magen pre-packed kits are designed for 32/48-channel automated nucleic acid extraction systems, providing ready-to-use reagent formats for magnetic bead–based purification workflows. These kits reduce manual reagent preparation and help laboratories standardize DNA and RNA extraction across routine sample processing.
Compared with large 96-channel formats, 32/48-channel pre-packed kits provide flexible throughput for laboratories handling moderate sample volumes or mixed workflow requirements. The product range supports automated purification from whole blood, buffy coat, tissue, cells, low cell-content biological samples, plasma, serum and body fluids.
By integrating MagPure magnetic bead chemistry with automation-ready reagent formats, these kits support consistent binding, washing and elution steps across compatible 32/48-channel extraction instruments.
Precast kits are designed to simplify automated nucleic acid extraction by providing pre-configured reagent formats for 96-channel extraction systems. By reducing manual reagent preparation and supporting standardized magnetic bead processing, these kits help laboratories improve workflow consistency, throughput and operational efficiency.
Magen MagPure precast kits are developed for automated DNA and RNA purification workflows across different sample types, including whole blood, plant materials, tissue, cells, low cell-content biological samples and plasma or serum. Each format is matched to a specific automated extraction workflow, supporting binding, washing and elution steps on compatible 96-channel instruments.
These kits are suitable for laboratories that require scalable, repeatable and easy-to-operate nucleic acid preparation workflows using magnetic bead technology.
Spin column–based nucleic acid purification is widely used for DNA and RNA extraction due to its consistency and ease of use. The method is based on selective binding of nucleic acids to a silica or glass fiber matrix, followed by washing and elution.
The HiPure spin column system is organized as a structured platform covering different sample input levels, from low-input purification to large-scale DNA preparation. Column selection depends primarily on sample input volume, expected yield, and workflow requirements. Detailed specifications are provided in the product-specific Purchase Guide.
This approach enables predictable and scalable nucleic acid purification across different laboratory workflows.
Chemical reagents form the foundation of nucleic acid extraction systems, controlling cell lysis, protein denaturation, inhibitor removal and nucleic acid binding conditions. The performance of DNA and RNA purification workflows depends not only on column or magnetic bead systems, but also on the composition and quality of these core reagents.
Magen molecular biology–grade reagents are selected and quality-controlled for integration into nucleic acid workflows. Chaotropic salts such as guanidine compounds enable protein denaturation and nucleic acid binding, while detergents and surfactants support membrane disruption. Additional components are used to remove inhibitors such as polyphenols and polysaccharides, improving extraction performance from challenging samples.
By optimizing these chemical components, stable and reproducible workflows can be achieved across applications including genomic DNA extraction, RNA purification and complex sample processing.
Enzymatic processing is a core component of nucleic acid sample preparation workflows, enabling efficient lysis, protein digestion and removal of interfering biomolecules prior to purification.
Within Magen systems, enzymes are not used as standalone reagents but as integrated workflow components supporting column and magnetic bead–based purification. Proteinase K enables effective protein digestion during lysis, while RNase A and DNase I are applied to selectively remove unwanted nucleic acids. In low-input workflows, carrier RNA improves recovery by reducing adsorption loss and stabilizing nucleic acids during processing.
These enzymatic steps ensure consistent nucleic acid release, improved purity and reliable downstream performance across diverse sample types including tissue, blood, microbial samples and low-abundance inputs.
The Viral & Pathogen series from Magen supports nucleic acid preparation workflows commonly used in infectious disease research and laboratory monitoring.
These systems are designed for low-cell biological samples such as plasma, serum, swab transport media and culture supernatants, as well as more complex matrices including sputum and tissue homogenates.
Two technical platforms are available within the series:
For routine viral nucleic acid extraction from low-background samples, column systems such as HiPure Viral DNA/RNA Kit (IVD4175) are commonly used. Laboratories using automated extraction platforms may prefer magnetic bead systems such as MagPure Viral DNA/RNA Kit (IVD5412).
For more complex microbial samples or sequencing-oriented pathogen studies, broader pathogen recovery systems are available, including HiPure Pathogen DNA/RNA Kit (IVD4179) for column workflows and MagPure Pathogen DNA/RNA Enrich Kit (R6672C) designed to improve pathogen signal in sequencing preparation workflows.
Purification of microRNA and other small RNA molecules requires extraction systems capable of efficiently recovering short RNA fragments while minimizing loss during purification steps. Reliable recovery of small RNAs is essential for downstream workflows including small RNA sequencing and regulatory RNA studies.
Magen provides small RNA extraction systems based on optimized column purification chemistry and complementary RNA purification workflows capable of recovering both total RNA and microRNA fractions from biological samples including tissues and cultured cells.
Within this system, the HiPure Universal miRNA Kit (R4310) enables enrichment and separation of microRNA and larger RNA molecules from biological samples. Laboratories requiring purification of both total RNA and microRNA may also use the HiPure Liquid RNA (miRNA) Kit (R4163) or the HiPure Universal RNA Kit (R4130), which support purification of RNA together with microRNA fractions for downstream gene expression and small RNA analysis workflows.
Microbial RNA preparation is strongly influenced by sample type. Soil, stool, bacterial cultures and other environmental materials differ in cell wall structure, inhibitor background and RNA stability, requiring tailored extraction strategies for reliable RNA recovery.
Environmental samples, particularly soil and stool, often contain polysaccharides, humic substances and other contaminants that can interfere with RNA purification and compromise downstream analysis. Effective workflows therefore require both efficient cell disruption and controlled background removal to preserve RNA integrity.
Magen microbial RNA systems cover a range of sample-specific solutions, including soil, stool, bacterial and yeast RNA extraction. The workflows integrate mechanical lysis with optimized purification chemistry designed to reduce inhibitor interference while maintaining RNA stability, supporting consistent performance across variable environmental samples.
Both spin column and magnetic bead formats are available to support routine laboratory workflows as well as automated and high-throughput processing.
Select the appropriate system below based on your sample type and workflow requirements.
RNA purification from whole blood requires careful control of RNase activity and removal of inhibitory components such as hemoglobin and globin RNA. Reliable extraction systems are therefore required to maintain RNA integrity for downstream gene expression analysis and transcriptomics workflows.
Magen provides blood RNA extraction systems designed for both manual centrifuge workflows and automated magnetic bead purification platforms. These systems support RNA purification from fresh blood samples as well as preserved or frozen blood materials used in molecular analysis.
Within this system, the MagPure Blood RNA Kit (R6611) serves as the magnetic bead reference configuration for automated purification of RNA from whole blood. Laboratories performing column-based workflows may refer to the HiPure Blood RNA Kit (R4161) for routine RNA purification.
For workflows involving preserved or frozen blood samples, the MagPure Blood RNA Kit II (R6613) provides a workflow that does not require red blood cell lysis and supports RNA purification from fresh or frozen blood samples. Small-volume or low-input samples may also be processed using the MagPure Blood RNA Kit C (R6611C).
Plant RNA extraction is often complicated by polysaccharides, polyphenols and active RNases that affect RNA integrity and downstream analysis. Effective workflows require both rapid RNase inactivation and controlled removal of inhibitory compounds.
Magen plant RNA extraction systems integrate column and magnetic bead workflows to support different plant sample types and experimental requirements.
For complex or variable plant samples, including tissues rich in polyphenols and polysaccharides, optimized guanidine-based lysis combined with antioxidant protection improves RNA stability and inhibitor control. A representative system is the HiPure Plant RNA Plus Kit (R4150), which uses dual-column purification to improve RNA quality for transcriptomic applications.
For difficult or inhibitor-rich samples where standard methods result in low yield or purity, CTAB-based workflows are applied to enhance impurity removal. The HiPure HP Plant RNA Mini Kit (R4165) represents this approach, combining CTAB lysis, organic extraction and DNase digestion for improved RNA purity.
For routine plant tissues, simplified guanidine-based workflows enable efficient RNA purification for RT-PCR and gene expression analysis.
Magnetic bead–based systems extend plant RNA extraction into automated and high-throughput workflows with consistent recovery across samples.
For fruit or starch-rich tissues, phenol-based workflows remain effective for challenging sample matrices.
Laboratories can select appropriate workflows based on sample composition, throughput and downstream application requirements.
RNA purification from tissues and cultured cells requires efficient disruption of cellular structures while maintaining RNA stability throughout the extraction workflow. Reliable purification is essential for downstream applications including RT-PCR analysis, gene expression profiling and transcriptomics studies.
Magen provides tissue RNA extraction systems based on both silica membrane spin column technology and magnetic bead purification platforms. These systems allow laboratories to perform RNA purification using either manual centrifuge workflows or automated magnetic extraction instruments.
Within this system, the HiPure Total RNA Plus Kit (R4111) serves as the column-based reference configuration incorporating genomic DNA removal to improve RNA purity. For automated laboratory environments, the MagPure Universal RNA Kit (IVD3020) provides the magnetic bead workflow designed for automated RNA purification from tissues and cultured cells. Broader sample compatibility can be achieved using the MagPure Universal RNA Kit II (R6623), while routine column purification workflows may use the HiPure Total RNA Kit (R4011).
DNA and RNA cleanup is a routine step in molecular workflows, used to remove primers, enzymes, salts and other reaction components prior to downstream applications such as PCR, cloning and sequencing.
Magen provides a complete cleanup system portfolio based on silica membrane and magnetic bead technologies. All systems are developed with a focus on consistent nucleic acid recovery, stable batch performance and compatibility with diverse laboratory workflows.
Compared to extraction systems, cleanup workflows follow a similar binding principle, and selection is typically determined by sample source and processing format rather than fundamental chemistry differences.
Agarose Gel Extraction
Designed for recovery of DNA fragments from agarose gels following electrophoresis.
PCR / Enzymatic Reaction Cleanup
Used for removal of primers, nucleotides, enzymes and salts from amplification and digestion reactions.
General DNA Cleanup
Provides flexible purification and concentration of DNA across different reaction systems.
RNA Cleanup
Designed for RNA desalting, concentration and purification while maintaining RNA integrity.
For workflows requiring fragment size selection or adjustable clean-up conditions in NGS library preparation, magnetic bead-based systems offer greater flexibility than fixed clean-up kits. Related products can be found in Magen NGS Clean-Up Magnetic Bead Systems.
Microbial DNA preparation is strongly influenced by sample type. Soil, stool, bacterial cultures and other environmental materials differ in cell wall structure, inhibitor background and biomass distribution, requiring different extraction strategies for reliable DNA recovery.
Environmental samples, particularly soil and stool, often contain humic substances, polysaccharides and bile-derived compounds that can interfere with downstream enzymatic reactions. Effective workflows therefore require both efficient cell disruption and consistent control of inhibitor background to ensure reliable downstream performance.
Magen microbial DNA systems cover a range of sample-specific solutions, including soil, stool, bacterial and yeast DNA extraction. The workflows integrate mechanical lysis with adsorbent-based cleanup designed to reduce inhibitor interference while maintaining nucleic acid recovery, supporting consistent performance across variable environmental samples.
Both spin column and magnetic bead formats are available to support routine laboratory workflows as well as automated and high-throughput processing.
Select the appropriate system below based on your sample type and workflow requirements.
Plant DNA extraction is often affected by polysaccharides, polyphenols and other secondary metabolites that interfere with downstream PCR and sequencing. Effective workflows require both efficient lysis and controlled removal of these inhibitors.
Magen plant DNA extraction systems integrate column and magnetic bead platforms to support different sample types and throughput requirements.
For complex plant samples such as polyphenol-rich or inhibitor-heavy tissues, CTAB-based workflows are typically applied to improve impurity removal and DNA stability. A representative product is the HiPure HP Plant DNA Kit (D3187).
For routine plant tissues with lower inhibitor content, detergent-based lysis enables rapid DNA purification with simplified handling, supporting standard PCR and genotyping workflows.
Magnetic bead–based systems extend plant DNA extraction into automated and high-throughput environments. The MagPure Plant DNA Kit (D6351) represents this workflow.
For seed samples, dedicated workflows improve lysis efficiency and impurity control for agricultural genomics applications.
Laboratories can select appropriate workflows based on sample complexity, throughput and automation requirements.
Genomic DNA purification from tissues and heterogeneous biological samples requires efficient cell disruption and removal of proteins, lipids and other inhibitory components. Reliable extraction performance is essential for downstream workflows including PCR amplification, genotyping studies and HLA typing.
Magen provides tissue and universal DNA extraction systems based on both silica membrane spin column technology and magnetic bead purification platforms. These systems support purification from a wide range of biological samples including animal tissues, cultured cells, blood derivatives and other laboratory materials.
Within this system, the HiPure Universal DNA Kit (D3018) serves as the column-based reference configuration for genomic DNA purification from diverse biological samples. For laboratories operating automated purification platforms, the MagPure Universal DNA Kit (IVD3102) provides the magnetic bead workflow designed for automated extraction systems. Tissue-based workflows may also refer to the HiPure Tissue DNA Kit (D3121) for routine genomic DNA purification from tissue samples.
For forensic DNA workflows, specialized extraction systems are available to support trace DNA recovery from challenging materials. The HiPure Bone DNA Kit (D3124) and MagPure Forensic DNA Kit (D6359D) are designed for purification of genomic DNA from bone, hair and other forensic samples requiring efficient recovery from low-input or degraded materials.
Genomic DNA extraction from whole blood requires efficient removal of inhibitors such as hemoglobin and anticoagulants while maintaining stable DNA recovery for downstream molecular analysis. Reliable purification is therefore essential for workflows including PCR amplification, genotyping studies and HLA typing.
Magen provides blood DNA extraction systems combining silica membrane spin column technology and magnetic bead purification platforms. These systems support both centrifuge-based laboratory workflows and automated magnetic extraction instruments.
Within this system, the HiPure Blood DNA Mini Kit (D3111) serves as the column-based reference configuration for routine genomic DNA purification from whole blood. For laboratories operating automated extraction workflows, the MagPure Blood DNA Kit (D6311) provides the magnetic bead platform designed for automated purification systems.
For laboratories processing multiple biological sample types, the HiPure Universal DNA Kit (D3018) and the automated MagPure Universal DNA Kit (IVD3102) also support genomic DNA purification from whole blood together with other biological samples, allowing universal extraction workflows across different sample types.
Formalin-fixed paraffin-embedded (FFPE) tissues are among the most widely archived biological materials used in oncology research and retrospective molecular studies. Extraction of nucleic acids from these samples requires efficient reversal of formaldehyde crosslinking and recovery of fragmented DNA or RNA suitable for downstream molecular analysis.
Magen provides a set of FFPE nucleic acid extraction systems developed for these workflows. The portfolio integrates both silica membrane column technology and magnetic bead purification chemistry to support different laboratory processing formats and sample throughput requirements.
Within this platform, column-based workflows support controlled purification of genomic DNA from FFPE tissue sections, while magnetic bead technologies enable scalable nucleic acid recovery and automation-compatible processing.
Representative FFPE purification systems include:
FFPE purification systems are available for multiple nucleic acid targets, including:
For a route-level overview of Magen FFPE DNA, RNA and DNA/RNA co-extraction workflows:
👉 FFPE Nucleic Acid Extraction Workflows Explained
For product-level selection based on target analyte, purification format and downstream application:
👉 FFPE Nucleic Acid Extraction Purchase Guide
The products listed below focus on genomic DNA extraction from FFPE tissue sections.
Circulating DNA extraction from plasma or serum requires efficient recovery of short cfDNA fragments while minimizing background genomic DNA contamination. Magen provides a set of circulating DNA extraction systems developed for these workflows, combining silica membrane column technology and magnetic bead-based purification to accommodate different plasma input volumes and laboratory processing formats.
Within this system, the HiPure Circulating DNA Kit (IVD3182) represents the column-based configuration designed for controlled vacuum or centrifuge workflows with plasma inputs up to 5 mL. The MagPure Circulating DNA Maxi Kit (IVD5435) serves as the magnetic bead platform for higher-volume plasma processing and automation-compatible workflows. Together these systems form the core extraction technologies within the Magen circulating DNA portfolio.
For a broader view of how these technologies fit into the Magen cfDNA workflow system, including route differences, processing logic and downstream application orientation, see the cfDNA Extraction & Enrichment Workflows page in our Technical Solutions section.
Related cfDNA Application Notes: These technical notes explain how Magen circulating DNA extraction systems connect to different plasma cfDNA research applications, including oncology ctDNA, methylation biomarkers, fragmentomics and prenatal cfDNA workflows.
Application Map · Oncology ctDNA · Methylation Biomarkers · Fragmentomics & MCED · Prenatal cfDNA
Co-isolation of DNA and RNA enables simultaneous analysis of genomic and transcriptomic information from a single sample, reducing sample consumption and improving workflow efficiency. This approach is particularly valuable in applications where sample input is limited or where direct comparison between DNA and RNA is required.
In nucleic acid extraction workflows, different co-isolation strategies are applied depending on sample type and downstream requirements. Some systems separate DNA and RNA during early lysis steps to preserve RNA integrity, while others rely on sequential binding and elution processes to maximize total nucleic acid recovery. These differences influence RNA quality, DNA yield and overall workflow flexibility.
Magen co-isolation systems are designed to support both column-based and magnetic bead–based workflows across a range of sample types including FFPE tissues, environmental samples, fibrous tissues and standard biological materials. By integrating optimized lysis chemistry and purification strategies, these systems enable reliable recovery of DNA and RNA for downstream applications such as sequencing, PCR and transcriptomic analysis.
For a route-level overview of Magen FFPE DNA, RNA and DNA/RNA co-extraction workflows:
👉 FFPE Nucleic Acid Extraction Workflows Explained
For product-level selection based on target analyte, purification format and downstream application:
👉 FFPE Nucleic Acid Extraction Purchase Guide
Automated nucleic acid extraction systems are designed to improve consistency, throughput and reproducibility in DNA and RNA purification workflows. By integrating magnetic bead–based purification with programmable processing steps, these systems enable standardized sample handling across a wide range of applications.
Magen extraction systems support flexible throughput configurations, from small-batch processing to high-throughput workflows, accommodating different laboratory requirements. These systems are compatible with multi-channel plate formats and magnetic bead consumables, enabling efficient binding, washing and elution processes in automated workflows.
Depending on application needs, different system configurations are available for low-volume, medium-volume and large-volume sample processing, including circulating nucleic acid workflows, pathogen detection, plant and tissue extraction and forensic applications. Auxiliary equipment further supports sample homogenization and liquid handling integration.
By combining automation systems with optimized reagents, consumables and workflow design, Magen provides a complete solution for nucleic acid sample preparation.
Sample preservation reagents play a critical role in maintaining nucleic acid integrity prior to extraction. During collection and transport, biological samples are exposed to nuclease activity, cellular degradation and environmental variation, which can significantly impact downstream performance.
Magen preservation reagents are designed to rapidly inactivate nucleases and stabilize nucleic acids at the point of collection. Different formulations are optimized for specific sample types, including solid tissues, liquid samples and complex matrices such as stool. These reagents support flexible storage conditions, enabling short-term room temperature transport and longer-term storage under refrigerated or frozen conditions.
By stabilizing DNA and RNA before extraction, these reagents improve consistency in downstream column and magnetic bead–based purification workflows, particularly in applications involving low-abundance or degradation-sensitive targets.
Sample stabilization is a critical upstream step in nucleic acid workflows, directly affecting downstream extraction efficiency and data reliability. During collection and transport, biological samples are exposed to nuclease activity, cellular degradation and environmental variation, which can lead to loss of DNA or RNA integrity.
Magen sample collection and preservation systems are designed to maintain nucleic acid stability across different sample types including tissue, blood, saliva, swabs and microbial samples. By controlling nuclease activity and stabilizing target molecules at the point of collection, these systems support consistent performance in downstream column and magnetic bead–based extraction workflows.
Different preservation strategies are applied depending on sample type and application, including genomic DNA stabilization, RNA protection for gene expression analysis and circulating nucleic acid preservation for liquid biopsy workflows.
Magnetic bead-based clean-up systems are widely used in post-reaction nucleic acid workflows, particularly for PCR product purification, fragment size selection and sequencing reaction clean-up. These processes help remove residual primers, enzymes, salts and other reaction components prior to downstream analysis.
Within the Magen portfolio, clean-up bead systems are developed based on PEG-mediated binding chemistry, enabling selective adsorption of nucleic acids under controlled conditions. Depending on bead configuration and workflow parameters, these systems support routine DNA/RNA purification as well as more specialized applications such as fragment size control in NGS library preparation.
Different bead configurations are optimized for distinct workflow stages, including routine clean-up, selective removal of short fragments, RNA purification and sequencing reaction purification. This allows laboratories to build flexible and reproducible workflows across molecular biology and sequencing applications while maintaining compatibility with both manual handling and automated platforms.
Magnetic bead materials provide a flexible platform for nucleic acid purification across a wide range of laboratory workflows, particularly where scalability, automation compatibility and adjustable binding conditions are required.
Within the Magen sample preparation portfolio, magnetic beads are engineered with defined surface chemistries, particle size distributions and magnetic response profiles to support DNA and RNA binding under chaotropic or PEG-mediated conditions. Silica-based particles are commonly used for nucleic acid extraction from routine, low-input or fragmented samples, while carboxyl-coated beads are applied in clean-up, size selection and enzymatic workflows such as NGS library preparation.
Different bead types are optimized for specific processing conditions, including sample input volume, nucleic acid abundance and fragment characteristics. This enables laboratories to select appropriate materials for applications ranging from general DNA/RNA isolation to circulating DNA workflows and post-extraction processing.
All magnetic bead systems are compatible with both manual protocols and automated platforms, supporting consistent performance across diverse laboratory environments.
Automated nucleic acid extraction systems rely on standardized consumables to ensure reproducibility and throughput. Deep well plates and tip combs serve as essential components in magnetic bead–based workflows, enabling liquid handling, binding, washing and elution steps across multi-channel platforms.
Magen consumables are designed for compatibility with common automated extraction systems, including 32-, 48- and 96-channel platforms. Manufactured from high-quality polypropylene and tested for DNase/RNase-free conditions, these products support reliable DNA and RNA purification while minimizing contamination risk.
Different plate formats and well geometries are available to accommodate various workflow requirements, including sample processing, storage and elution. Tip comb systems enable efficient magnetic bead transfer and washing during automated extraction procedures.
Plasmid DNA preparation is selected mainly by workflow requirements rather than sample type. In practical laboratory work, the key factors usually include plasmid copy number, endotoxin requirement, culture input, and whether the workflow is manual, high-throughput, or automation-oriented.
Magen provides a structured plasmid DNA extraction portfolio based on column and magnetic bead systems, covering both standard plasmid preparation and low-endotoxin workflows for higher-purity applications. Different products in the series are positioned for different culture inputs and downstream requirements, from routine small-scale preparation to larger-volume processing, parallel batch handling and automation-compatible workflows.
Within the current plasmid system, HiPure Plasmid Mini Kit (P1001) serves as the standard column reference product for routine plasmid preparation, while HiPure Plasmid EF Maxi Kit B (P1156B) functions as the primary low-endotoxin column reference model for larger and more demanding EF workflows. Other products in the series extend this system toward faster clarification, broader low-endotoxin preparation, high-throughput handling, or magnetic bead-based processing.
For workflow-based product selection across standard and low-endotoxin plasmid preparation, please refer to the Plasmid DNA Kits Purchase Guide.
Mechanical lysis is a critical step in nucleic acid extraction, particularly for samples that are difficult to disrupt such as microbial cells, plant tissues and complex biological matrices. Bead-based homogenization uses controlled mechanical force to break cell walls and release nucleic acids for downstream purification.
Magen bead tubes integrate optimized homogenization beads and tube formats into a ready-to-use system for different sample types. By combining specific bead materials, sizes and compositions, these systems enable efficient disruption of bacterial, fungal, tissue and stool samples while reducing variability between experiments.
Different homogenization beads provide distinct mechanical properties: smaller beads increase surface contact for microbial lysis, while larger or high-density materials generate stronger impact for tough tissues. Pre-filled bead tubes further standardize workflows by eliminating manual bead selection and improving reproducibility across samples.
By integrating bead composition with workflow design, these systems support consistent upstream processing for column and magnetic bead–based nucleic acid extraction workflows.
High molecular weight DNA extraction requires a balance between efficient cell disruption and preservation of long DNA fragments. In practice, different sample types introduce distinct challenges: soft tissues are relatively easy to digest, while microbial and plant samples often require additional strategies to overcome cell wall structures or inhibitory compounds.
The Magen High Molecular Weight DNA system is primarily based on magnetic bead technology, allowing controlled binding and gentle handling conditions that help reduce DNA fragmentation during purification. The workflow integrates protease-based lysis, optimized binding chemistry, and multi-step washing to maintain DNA integrity while removing contaminants.
Within this system, different kits are designed for specific sample categories. Universal workflows support routine biological samples, while dedicated systems introduce targeted strategies such as enzymatic lysis for bacteria, bead-assisted disruption for fungi, and inhibitor control for plant tissues. This structured approach enables laboratories to match extraction conditions to sample characteristics without compromising DNA fragment length.
In addition to magnetic bead–based systems, Magen also provides salt-out–based DNA extraction solutions for laboratories requiring a simplified workflow and cost-controlled processing. These systems are typically used for routine genomic DNA preparation, while magnetic bead platforms are preferred when higher DNA integrity or automation compatibility is required.
The purified DNA generated from these workflows is suitable for applications where DNA integrity directly impacts performance. This includes long-read sequencing platforms such as nanopore-based analysis, genome assembly, and structural variation studies, where preservation of fragment length is critical for accurate downstream results.
Formalin-fixed paraffin-embedded (FFPE) tissues are among the most widely archived biological materials used in oncology research and retrospective molecular studies. Extraction of nucleic acids from these samples requires efficient reversal of formaldehyde crosslinking and recovery of fragmented DNA or RNA suitable for downstream molecular analysis.
Magen provides a set of FFPE nucleic acid extraction systems developed for these workflows. The portfolio integrates both silica membrane column technology and magnetic bead purification chemistry to support different laboratory processing formats and sample throughput requirements.
Within this platform, column-based workflows support controlled purification of genomic DNA from FFPE tissue sections, while magnetic bead technologies enable scalable nucleic acid recovery and automation-compatible processing.
Representative FFPE purification systems include:
FFPE purification systems are available for multiple nucleic acid targets, including:
For a route-level overview of Magen FFPE DNA, RNA and DNA/RNA co-extraction workflows:
👉 FFPE Nucleic Acid Extraction Workflows Explained
For product-level selection based on target analyte, purification format and downstream application:
👉 FFPE Nucleic Acid Extraction Purchase Guide
The products listed below focus on genomic RNA extraction from FFPE tissue sections.
Magen pre-packed kits are designed for 32/48-channel automated nucleic acid extraction systems, providing ready-to-use reagent formats for magnetic bead–based purification workflows. These kits reduce manual reagent preparation and help laboratories standardize DNA and RNA extraction across routine sample processing.
Compared with large 96-channel formats, 32/48-channel pre-packed kits provide flexible throughput for laboratories handling moderate sample volumes or mixed workflow requirements. The product range supports automated purification from whole blood, buffy coat, tissue, cells, low cell-content biological samples, plasma, serum and body fluids.
By integrating MagPure magnetic bead chemistry with automation-ready reagent formats, these kits support consistent binding, washing and elution steps across compatible 32/48-channel extraction instruments.
Precast kits are designed to simplify automated nucleic acid extraction by providing pre-configured reagent formats for 96-channel extraction systems. By reducing manual reagent preparation and supporting standardized magnetic bead processing, these kits help laboratories improve workflow consistency, throughput and operational efficiency.
Magen MagPure precast kits are developed for automated DNA and RNA purification workflows across different sample types, including whole blood, plant materials, tissue, cells, low cell-content biological samples and plasma or serum. Each format is matched to a specific automated extraction workflow, supporting binding, washing and elution steps on compatible 96-channel instruments.
These kits are suitable for laboratories that require scalable, repeatable and easy-to-operate nucleic acid preparation workflows using magnetic bead technology.
La lisis mecánica es una etapa fundamental de la extracción de ácidos nucleicos, especialmente en muestras difíciles de disgregar, como células microbianas, tejidos vegetales y matrices biológicas complejas. La homogeneización con perlas utiliza fuerzas mecánicas controladas para romper las paredes celulares y liberar los ácidos nucleicos antes de su purificación.
Los tubos con perlas Magen integran perlas de homogeneización optimizadas y distintos formatos de tubo en sistemas listos para usar con diversos tipos de muestras. La combinación de materiales, tamaños y composiciones específicos permite una disrupción eficaz de muestras bacterianas, fúngicas, tisulares y fecales, al tiempo que reduce la variabilidad entre ensayos.
Las distintas perlas de homogeneización presentan propiedades mecánicas específicas: las perlas más pequeñas aumentan la superficie de contacto para la lisis microbiana, mientras que las de mayor tamaño o fabricadas con materiales de alta densidad generan un impacto más intenso para disgregar tejidos resistentes. Los tubos precargados con perlas también estandarizan los flujos de trabajo al evitar la selección y adición manual de las perlas y mejorar la reproducibilidad entre muestras.
Mediante la integración de la composición de las perlas en el diseño del flujo de trabajo, estos sistemas permiten un procesamiento inicial uniforme para la extracción de ácidos nucleicos mediante columnas o partículas magnéticas.
Los reactivos químicos constituyen la base de los sistemas de extracción de ácidos nucleicos, ya que controlan la lisis celular, la desnaturalización de proteínas, la eliminación de inhibidores y las condiciones de unión de los ácidos nucleicos. El rendimiento de los flujos de trabajo de purificación de ADN y ARN depende no solo de los sistemas de columnas o partículas magnéticas, sino también de la composición y la calidad de estos reactivos esenciales.
Los reactivos Magen de grado biología molecular se seleccionan y someten a controles de calidad para su integración en flujos de trabajo de extracción de ácidos nucleicos. Las sales caotrópicas, como los compuestos de guanidinio, favorecen la desnaturalización de proteínas y la unión de los ácidos nucleicos, mientras que los detergentes y tensioactivos facilitan la disrupción de las membranas. Otros componentes permiten eliminar inhibidores como polifenoles y polisacáridos, mejorando el rendimiento de la extracción a partir de muestras complejas.
La optimización de estos componentes químicos permite obtener flujos de trabajo estables y reproducibles en aplicaciones como la extracción de ADN genómico, la purificación de ARN y el procesamiento de muestras complejas.
El tratamiento enzimático es un componente fundamental de los flujos de trabajo de preparación de muestras para la extracción de ácidos nucleicos, ya que facilita una lisis eficaz, la digestión de proteínas y la eliminación de biomoléculas interferentes antes de la purificación.
En los sistemas Magen, las enzimas no se utilizan como reactivos independientes, sino como componentes integrados en flujos de trabajo de purificación basados en columnas y partículas magnéticas. La proteinasa K permite una digestión eficaz de las proteínas durante la lisis, mientras que la RNasa A y la DNasa I se emplean para eliminar selectivamente el ARN o el ADN no deseados, respectivamente. En los flujos de trabajo con cantidades reducidas de muestra, el ARN transportador mejora la recuperación al reducir las pérdidas por adsorción y estabilizar los ácidos nucleicos durante el procesamiento.
Estas etapas favorecen una liberación uniforme de los ácidos nucleicos, una mayor pureza y un rendimiento fiable en las aplicaciones posteriores para diversos tipos de muestras, incluidos tejidos, sangre, muestras microbianas y materiales con baja abundancia de ácidos nucleicos.
La purificación de ácidos nucleicos mediante columnas de centrifugación se utiliza ampliamente para la extracción de ADN y ARN por su reproducibilidad y facilidad de uso. El método se basa en la unión selectiva de los ácidos nucleicos a una matriz de sílice o fibra de vidrio, seguida de etapas de lavado y elución.
El sistema de columnas de centrifugación HiPure constituye una plataforma estructurada que abarca distintos niveles de entrada de muestra, desde la purificación de cantidades reducidas hasta la preparación de ADN a gran escala. La selección de la columna depende principalmente de la cantidad o el volumen de muestra, el rendimiento previsto y los requisitos del flujo de trabajo. Las especificaciones detalladas se encuentran en la guía de selección correspondiente a cada producto.
Este enfoque permite una purificación de ácidos nucleicos reproducible y escalable en distintos flujos de trabajo de laboratorio.
Purification of microRNA and other small RNA molecules requires extraction systems capable of efficiently recovering short RNA fragments while minimizing loss during purification steps. Reliable recovery of small RNAs is essential for downstream workflows including small RNA sequencing and regulatory RNA studies.
Magen provides small RNA extraction systems based on optimized column purification chemistry and complementary RNA purification workflows capable of recovering both total RNA and microRNA fractions from biological samples including tissues and cultured cells.
Within this system, the HiPure Universal miRNA Kit (R4310) enables enrichment and separation of microRNA and larger RNA molecules from biological samples. Laboratories requiring purification of both total RNA and microRNA may also use the HiPure Liquid RNA (miRNA) Kit (R4163) or the HiPure Universal RNA Kit (R4130), which support purification of RNA together with microRNA fractions for downstream gene expression and small RNA analysis workflows.
The Viral & Pathogen series from Magen supports nucleic acid preparation workflows commonly used in infectious disease research and laboratory monitoring.
These systems are designed for low-cell biological samples such as plasma, serum, swab transport media and culture supernatants, as well as more complex matrices including sputum and tissue homogenates.
Two technical platforms are available within the series:
For routine viral nucleic acid extraction from low-background samples, column systems such as HiPure Viral DNA/RNA Kit (IVD4175) are commonly used. Laboratories using automated extraction platforms may prefer magnetic bead systems such as MagPure Viral DNA/RNA Kit (IVD5412).
For more complex microbial samples or sequencing-oriented pathogen studies, broader pathogen recovery systems are available, including HiPure Pathogen DNA/RNA Kit (IVD4179) for column workflows and MagPure Pathogen DNA/RNA Enrich Kit (R6672C) designed to improve pathogen signal in sequencing preparation workflows.
Microbial RNA preparation is strongly influenced by sample type. Soil, stool, bacterial cultures and other environmental materials differ in cell wall structure, inhibitor background and RNA stability, requiring tailored extraction strategies for reliable RNA recovery.
Environmental samples, particularly soil and stool, often contain polysaccharides, humic substances and other contaminants that can interfere with RNA purification and compromise downstream analysis. Effective workflows therefore require both efficient cell disruption and controlled background removal to preserve RNA integrity.
Magen microbial RNA systems cover a range of sample-specific solutions, including soil, stool, bacterial and yeast RNA extraction. The workflows integrate mechanical lysis with optimized purification chemistry designed to reduce inhibitor interference while maintaining RNA stability, supporting consistent performance across variable environmental samples.
Both spin column and magnetic bead formats are available to support routine laboratory workflows as well as automated and high-throughput processing.
Select the appropriate system below based on your sample type and workflow requirements.
Plant RNA extraction is often complicated by polysaccharides, polyphenols and active RNases that affect RNA integrity and downstream analysis. Effective workflows require both rapid RNase inactivation and controlled removal of inhibitory compounds.
Magen plant RNA extraction systems integrate column and magnetic bead workflows to support different plant sample types and experimental requirements.
For complex or variable plant samples, including tissues rich in polyphenols and polysaccharides, optimized guanidine-based lysis combined with antioxidant protection improves RNA stability and inhibitor control. A representative system is the HiPure Plant RNA Plus Kit (R4150), which uses dual-column purification to improve RNA quality for transcriptomic applications.
For difficult or inhibitor-rich samples where standard methods result in low yield or purity, CTAB-based workflows are applied to enhance impurity removal. The HiPure HP Plant RNA Mini Kit (R4165) represents this approach, combining CTAB lysis, organic extraction and DNase digestion for improved RNA purity.
For routine plant tissues, simplified guanidine-based workflows enable efficient RNA purification for RT-PCR and gene expression analysis.
Magnetic bead–based systems extend plant RNA extraction into automated and high-throughput workflows with consistent recovery across samples.
For fruit or starch-rich tissues, phenol-based workflows remain effective for challenging sample matrices.
Laboratories can select appropriate workflows based on sample composition, throughput and downstream application requirements.
Formalin-fixed paraffin-embedded (FFPE) tissues are among the most widely archived biological materials used in oncology research and retrospective molecular studies. Extraction of nucleic acids from these samples requires efficient reversal of formaldehyde crosslinking and recovery of fragmented DNA or RNA suitable for downstream molecular analysis.
Magen provides a set of FFPE nucleic acid extraction systems developed for these workflows. The portfolio integrates both silica membrane column technology and magnetic bead purification chemistry to support different laboratory processing formats and sample throughput requirements.
Within this platform, column-based workflows support controlled purification of genomic DNA from FFPE tissue sections, while magnetic bead technologies enable scalable nucleic acid recovery and automation-compatible processing.
Representative FFPE purification systems include:
FFPE purification systems are available for multiple nucleic acid targets, including:
For a route-level overview of Magen FFPE DNA, RNA and DNA/RNA co-extraction workflows:
👉 FFPE Nucleic Acid Extraction Workflows Explained
For product-level selection based on target analyte, purification format and downstream application:
👉 FFPE Nucleic Acid Extraction Purchase Guide
The products listed below focus on genomic RNA extraction from FFPE tissue sections.
RNA purification from whole blood requires careful control of RNase activity and removal of inhibitory components such as hemoglobin and globin RNA. Reliable extraction systems are therefore required to maintain RNA integrity for downstream gene expression analysis and transcriptomics workflows.
Magen provides blood RNA extraction systems designed for both manual centrifuge workflows and automated magnetic bead purification platforms. These systems support RNA purification from fresh blood samples as well as preserved or frozen blood materials used in molecular analysis.
Within this system, the MagPure Blood RNA Kit (R6611) serves as the magnetic bead reference configuration for automated purification of RNA from whole blood. Laboratories performing column-based workflows may refer to the HiPure Blood RNA Kit (R4161) for routine RNA purification.
For workflows involving preserved or frozen blood samples, the MagPure Blood RNA Kit II (R6613) provides a workflow that does not require red blood cell lysis and supports RNA purification from fresh or frozen blood samples. Small-volume or low-input samples may also be processed using the MagPure Blood RNA Kit C (R6611C).
RNA purification from tissues and cultured cells requires efficient disruption of cellular structures while maintaining RNA stability throughout the extraction workflow. Reliable purification is essential for downstream applications including RT-PCR analysis, gene expression profiling and transcriptomics studies.
Magen provides tissue RNA extraction systems based on both silica membrane spin column technology and magnetic bead purification platforms. These systems allow laboratories to perform RNA purification using either manual centrifuge workflows or automated magnetic extraction instruments.
Within this system, the HiPure Total RNA Plus Kit (R4111) serves as the column-based reference configuration incorporating genomic DNA removal to improve RNA purity. For automated laboratory environments, the MagPure Universal RNA Kit (IVD3020) provides the magnetic bead workflow designed for automated RNA purification from tissues and cultured cells. Broader sample compatibility can be achieved using the MagPure Universal RNA Kit II (R6623), while routine column purification workflows may use the HiPure Total RNA Kit (R4011).
High molecular weight DNA extraction requires a balance between efficient cell disruption and preservation of long DNA fragments. In practice, different sample types introduce distinct challenges: soft tissues are relatively easy to digest, while microbial and plant samples often require additional strategies to overcome cell wall structures or inhibitory compounds.
The Magen High Molecular Weight DNA system is primarily based on magnetic bead technology, allowing controlled binding and gentle handling conditions that help reduce DNA fragmentation during purification. The workflow integrates protease-based lysis, optimized binding chemistry, and multi-step washing to maintain DNA integrity while removing contaminants.
Within this system, different kits are designed for specific sample categories. Universal workflows support routine biological samples, while dedicated systems introduce targeted strategies such as enzymatic lysis for bacteria, bead-assisted disruption for fungi, and inhibitor control for plant tissues. This structured approach enables laboratories to match extraction conditions to sample characteristics without compromising DNA fragment length.
In addition to magnetic bead–based systems, Magen also provides salt-out–based DNA extraction solutions for laboratories requiring a simplified workflow and cost-controlled processing. These systems are typically used for routine genomic DNA preparation, while magnetic bead platforms are preferred when higher DNA integrity or automation compatibility is required.
The purified DNA generated from these workflows is suitable for applications where DNA integrity directly impacts performance. This includes long-read sequencing platforms such as nanopore-based analysis, genome assembly, and structural variation studies, where preservation of fragment length is critical for accurate downstream results.
DNA and RNA cleanup is a routine step in molecular workflows, used to remove primers, enzymes, salts and other reaction components prior to downstream applications such as PCR, cloning and sequencing.
Magen provides a complete cleanup system portfolio based on silica membrane and magnetic bead technologies. All systems are developed with a focus on consistent nucleic acid recovery, stable batch performance and compatibility with diverse laboratory workflows.
Compared to extraction systems, cleanup workflows follow a similar binding principle, and selection is typically determined by sample source and processing format rather than fundamental chemistry differences.
Agarose Gel Extraction
Designed for recovery of DNA fragments from agarose gels following electrophoresis.
PCR / Enzymatic Reaction Cleanup
Used for removal of primers, nucleotides, enzymes and salts from amplification and digestion reactions.
General DNA Cleanup
Provides flexible purification and concentration of DNA across different reaction systems.
RNA Cleanup
Designed for RNA desalting, concentration and purification while maintaining RNA integrity.
For workflows requiring fragment size selection or adjustable clean-up conditions in NGS library preparation, magnetic bead-based systems offer greater flexibility than fixed clean-up kits. Related products can be found in Magen NGS Clean-Up Magnetic Bead Systems.
Microbial DNA preparation is strongly influenced by sample type. Soil, stool, bacterial cultures and other environmental materials differ in cell wall structure, inhibitor background and biomass distribution, requiring different extraction strategies for reliable DNA recovery.
Environmental samples, particularly soil and stool, often contain humic substances, polysaccharides and bile-derived compounds that can interfere with downstream enzymatic reactions. Effective workflows therefore require both efficient cell disruption and consistent control of inhibitor background to ensure reliable downstream performance.
Magen microbial DNA systems cover a range of sample-specific solutions, including soil, stool, bacterial and yeast DNA extraction. The workflows integrate mechanical lysis with adsorbent-based cleanup designed to reduce inhibitor interference while maintaining nucleic acid recovery, supporting consistent performance across variable environmental samples.
Both spin column and magnetic bead formats are available to support routine laboratory workflows as well as automated and high-throughput processing.
Select the appropriate system below based on your sample type and workflow requirements.
Plant DNA extraction is often affected by polysaccharides, polyphenols and other secondary metabolites that interfere with downstream PCR and sequencing. Effective workflows require both efficient lysis and controlled removal of these inhibitors.
Magen plant DNA extraction systems integrate column and magnetic bead platforms to support different sample types and throughput requirements.
For complex plant samples such as polyphenol-rich or inhibitor-heavy tissues, CTAB-based workflows are typically applied to improve impurity removal and DNA stability. A representative product is the HiPure HP Plant DNA Kit (D3187).
For routine plant tissues with lower inhibitor content, detergent-based lysis enables rapid DNA purification with simplified handling, supporting standard PCR and genotyping workflows.
Magnetic bead–based systems extend plant DNA extraction into automated and high-throughput environments. The MagPure Plant DNA Kit (D6351) represents this workflow.
For seed samples, dedicated workflows improve lysis efficiency and impurity control for agricultural genomics applications.
Laboratories can select appropriate workflows based on sample complexity, throughput and automation requirements.
Formalin-fixed paraffin-embedded (FFPE) tissues are among the most widely archived biological materials used in oncology research and retrospective molecular studies. Extraction of nucleic acids from these samples requires efficient reversal of formaldehyde crosslinking and recovery of fragmented DNA or RNA suitable for downstream molecular analysis.
Magen provides a set of FFPE nucleic acid extraction systems developed for these workflows. The portfolio integrates both silica membrane column technology and magnetic bead purification chemistry to support different laboratory processing formats and sample throughput requirements.
Within this platform, column-based workflows support controlled purification of genomic DNA from FFPE tissue sections, while magnetic bead technologies enable scalable nucleic acid recovery and automation-compatible processing.
Representative FFPE purification systems include:
FFPE purification systems are available for multiple nucleic acid targets, including:
For a route-level overview of Magen FFPE DNA, RNA and DNA/RNA co-extraction workflows:
👉 FFPE Nucleic Acid Extraction Workflows Explained
For product-level selection based on target analyte, purification format and downstream application:
👉 FFPE Nucleic Acid Extraction Purchase Guide
The products listed below focus on genomic DNA extraction from FFPE tissue sections.
Circulating DNA extraction from plasma or serum requires efficient recovery of short cfDNA fragments while minimizing background genomic DNA contamination. Magen provides a set of circulating DNA extraction systems developed for these workflows, combining silica membrane column technology and magnetic bead-based purification to accommodate different plasma input volumes and laboratory processing formats.
Within this system, the HiPure Circulating DNA Kit (IVD3182) represents the column-based configuration designed for controlled vacuum or centrifuge workflows with plasma inputs up to 5 mL. The MagPure Circulating DNA Maxi Kit (IVD5435) serves as the magnetic bead platform for higher-volume plasma processing and automation-compatible workflows. Together these systems form the core extraction technologies within the Magen circulating DNA portfolio.
For a broader view of how these technologies fit into the Magen cfDNA workflow system, including route differences, processing logic and downstream application orientation, see the cfDNA Extraction & Enrichment Workflows page in our Technical Solutions section.
Related cfDNA Application Notes: These technical notes explain how Magen circulating DNA extraction systems connect to different plasma cfDNA research applications, including oncology ctDNA, methylation biomarkers, fragmentomics and prenatal cfDNA workflows.
Application Map · Oncology ctDNA · Methylation Biomarkers · Fragmentomics & MCED · Prenatal cfDNA
Genomic DNA extraction from whole blood requires efficient removal of inhibitors such as hemoglobin and anticoagulants while maintaining stable DNA recovery for downstream molecular analysis. Reliable purification is therefore essential for workflows including PCR amplification, genotyping studies and HLA typing.
Magen provides blood DNA extraction systems combining silica membrane spin column technology and magnetic bead purification platforms. These systems support both centrifuge-based laboratory workflows and automated magnetic extraction instruments.
Within this system, the HiPure Blood DNA Mini Kit (D3111) serves as the column-based reference configuration for routine genomic DNA purification from whole blood. For laboratories operating automated extraction workflows, the MagPure Blood DNA Kit (D6311) provides the magnetic bead platform designed for automated purification systems.
For laboratories processing multiple biological sample types, the HiPure Universal DNA Kit (D3018) and the automated MagPure Universal DNA Kit (IVD3102) also support genomic DNA purification from whole blood together with other biological samples, allowing universal extraction workflows across different sample types.
La purificación de ADN genómico a partir de tejidos y muestras biológicas heterogéneas requiere una disrupción celular eficaz y la eliminación de proteínas, lípidos y otros componentes inhibidores. Un rendimiento de extracción fiable es esencial para aplicaciones posteriores, como la amplificación por PCR, los estudios de genotipado y la tipificación HLA.
Magen ofrece sistemas de extracción de ADN de tejidos y muestras diversas basados tanto en columnas de centrifugación con membrana de sílice como en plataformas de purificación con partículas magnéticas. Estos sistemas permiten purificar ADN a partir de una amplia variedad de muestras biológicas, como tejidos animales, células cultivadas, derivados sanguíneos y otros materiales de laboratorio.
Dentro de esta gama, el HiPure Universal DNA Kit (D3018) constituye la configuración de referencia basada en columnas para la purificación de ADN genómico a partir de diversas muestras biológicas. Para los laboratorios que utilizan plataformas de purificación automatizadas, el MagPure Universal DNA Kit (IVD3102) ofrece un flujo de trabajo con partículas magnéticas diseñado para sistemas de extracción automatizados. Para la purificación rutinaria de ADN genómico a partir de muestras de tejido, también puede utilizarse el HiPure Tissue DNA Kit (D3121).
Para los flujos de trabajo de ADN forense, se ofrecen sistemas de extracción especializados que favorecen la recuperación de ADN en indicios procedentes de materiales difíciles. El HiPure Bone DNA Kit (D3124) y el MagPure Forensic DNA Kit (D6359D) están diseñados para purificar ADN genómico a partir de huesos, cabellos y otras muestras forenses que requieren una recuperación eficaz de material escaso o degradado.
El coaislamiento de ADN y ARN permite analizar simultáneamente la información genómica y transcriptómica de una misma muestra, reduciendo el consumo de material y mejorando la eficiencia del flujo de trabajo. Este enfoque resulta especialmente valioso cuando la cantidad de muestra es limitada o se requiere una comparación directa entre el ADN y el ARN.
En los flujos de trabajo de extracción de ácidos nucleicos se aplican distintas estrategias de coaislamiento en función del tipo de muestra y de los requisitos de las aplicaciones posteriores. Algunos sistemas separan el ADN y el ARN durante las primeras etapas de lisis para preservar la integridad del ARN, mientras que otros emplean procesos secuenciales de unión y elución para maximizar la recuperación total de ácidos nucleicos. Estas diferencias influyen en la calidad del ARN, el rendimiento del ADN y la flexibilidad general del flujo de trabajo.
Los sistemas Magen de coaislamiento están diseñados para flujos de trabajo basados tanto en columnas como en partículas magnéticas y son compatibles con diversos tipos de muestras, incluidos tejidos FFPE, muestras ambientales, tejidos fibrosos y materiales biológicos convencionales. Mediante la integración de reactivos de lisis y estrategias de purificación optimizados, estos sistemas permiten recuperar ADN y ARN de manera fiable para aplicaciones posteriores como secuenciación, PCR y análisis transcriptómicos.
Para consultar una descripción general de las distintas rutas de extracción de ADN, ARN y coextracción de ADN/ARN a partir de muestras FFPE:
👉 Flujos de trabajo para la extracción de ácidos nucleicos de muestras FFPE
Para seleccionar productos según el analito objetivo, el formato de purificación y la aplicación posterior:
👉 Guía de selección para la extracción de ácidos nucleicos de muestras FFPE
Automated nucleic acid extraction systems are designed to improve consistency, throughput and reproducibility in DNA and RNA purification workflows. By integrating magnetic bead–based purification with programmable processing steps, these systems enable standardized sample handling across a wide range of applications.
Magen extraction systems support flexible throughput configurations, from small-batch processing to high-throughput workflows, accommodating different laboratory requirements. These systems are compatible with multi-channel plate formats and magnetic bead consumables, enabling efficient binding, washing and elution processes in automated workflows.
Depending on application needs, different system configurations are available for low-volume, medium-volume and large-volume sample processing, including circulating nucleic acid workflows, pathogen detection, plant and tissue extraction and forensic applications. Auxiliary equipment further supports sample homogenization and liquid handling integration.
By combining automation systems with optimized reagents, consumables and workflow design, Magen provides a complete solution for nucleic acid sample preparation.
Los reactivos para la conservación de muestras desempeñan un papel fundamental en el mantenimiento de la integridad de los ácidos nucleicos antes de la extracción. Durante la recogida y el transporte, las muestras biológicas están expuestas a la actividad de las nucleasas, la degradación celular y las variaciones ambientales, factores que pueden afectar significativamente al rendimiento de las aplicaciones posteriores.
Los reactivos de conservación Magen están diseñados para inactivar rápidamente las nucleasas y estabilizar los ácidos nucleicos desde el momento de la recogida. Las distintas formulaciones están optimizadas para tipos de muestra específicos, como tejidos sólidos, muestras líquidas y matrices complejas, entre ellas las muestras fecales. Estos reactivos permiten condiciones de almacenamiento flexibles, incluido el transporte a corto plazo a temperatura ambiente y la conservación durante periodos más prolongados en refrigeración o congelación.
Al estabilizar el ADN y el ARN antes de la extracción, estos reactivos mejoran la uniformidad de los flujos de trabajo posteriores de purificación mediante columnas o partículas magnéticas, especialmente en aplicaciones con dianas poco abundantes o sensibles a la degradación.
La estabilización de las muestras es una etapa inicial fundamental de los flujos de trabajo de ácidos nucleicos, ya que afecta directamente a la eficiencia de la extracción posterior y a la fiabilidad de los datos. Durante la recogida y el transporte, las muestras biológicas están expuestas a la actividad de las nucleasas, la degradación celular y las variaciones ambientales, lo que puede provocar la pérdida de integridad del ADN o el ARN.
Los sistemas Magen para la recogida y conservación de muestras están diseñados para mantener la estabilidad de los ácidos nucleicos en distintos tipos de muestras, incluidos tejidos, sangre, saliva, hisopos y muestras microbianas. Al controlar la actividad de las nucleasas y estabilizar las moléculas diana desde el momento de la recogida, estos sistemas favorecen un rendimiento uniforme en los flujos de trabajo posteriores de extracción mediante columnas o partículas magnéticas.
Se aplican distintas estrategias de conservación en función del tipo de muestra y de la aplicación, como la estabilización del ADN genómico, la protección del ARN para el análisis de la expresión génica y la conservación de ácidos nucleicos circulantes para los flujos de trabajo de biopsia líquida.
Los sistemas de limpieza basados en partículas magnéticas se utilizan ampliamente en los flujos de trabajo de procesamiento de ácidos nucleicos posteriores a una reacción, especialmente para la purificación de productos de PCR, la selección de fragmentos por tamaño y la limpieza de reacciones de secuenciación. Estos procesos permiten eliminar cebadores residuales, enzimas, sales y otros componentes de la reacción antes de los análisis posteriores.
Dentro de la gama Magen, los sistemas de partículas para limpieza se desarrollan a partir de una química de unión mediada por PEG, que permite la adsorción selectiva de ácidos nucleicos en condiciones controladas. En función de la configuración de las partículas y de los parámetros del flujo de trabajo, estos sistemas permiten tanto la purificación rutinaria de ADN y ARN como aplicaciones más especializadas, entre ellas el control del tamaño de los fragmentos durante la preparación de bibliotecas para NGS.
Las distintas configuraciones de partículas están optimizadas para etapas específicas del flujo de trabajo, como la limpieza rutinaria, la eliminación selectiva de fragmentos cortos, la purificación de ARN y la limpieza de reacciones de secuenciación. Esto permite a los laboratorios establecer flujos de trabajo flexibles y reproducibles para aplicaciones de biología molecular y secuenciación, manteniendo la compatibilidad tanto con el procesamiento manual como con las plataformas automatizadas.
Las partículas magnéticas proporcionan una plataforma flexible para la purificación de ácidos nucleicos en una amplia variedad de flujos de trabajo de laboratorio, especialmente cuando se requieren escalabilidad, compatibilidad con la automatización y condiciones de unión ajustables.
Dentro de la gama Magen para la preparación de muestras, las partículas magnéticas se desarrollan con químicas de superficie, distribuciones de tamaño y perfiles de respuesta magnética definidos para favorecer la unión de ADN y ARN en condiciones mediadas por agentes caotrópicos o PEG. Las partículas a base de sílice se utilizan habitualmente para extraer ácidos nucleicos de muestras rutinarias, con poca cantidad de material o con ácidos nucleicos fragmentados, mientras que las partículas carboxiladas se emplean en procesos de limpieza, selección por tamaño y flujos de trabajo enzimáticos, como la preparación de bibliotecas para NGS.
Los distintos tipos de partículas están optimizados para condiciones de procesamiento específicas, como el volumen de entrada de la muestra, la abundancia de ácidos nucleicos y las características de los fragmentos. Esto permite seleccionar los materiales adecuados para aplicaciones que abarcan desde el aislamiento general de ADN y ARN hasta los flujos de trabajo con ADN circulante y el procesamiento posterior a la extracción.
Todos los sistemas de partículas magnéticas son compatibles tanto con protocolos manuales como con plataformas automatizadas, lo que favorece un rendimiento uniforme en distintos entornos de laboratorio.
Los sistemas automatizados de extracción de ácidos nucleicos requieren consumibles estandarizados para garantizar la reproducibilidad y la capacidad de procesamiento. Las placas de pocillos profundos y los peines de puntas son componentes esenciales de los flujos de trabajo basados en partículas magnéticas, ya que permiten realizar las etapas de manipulación de líquidos, unión, lavado y elución en plataformas multicanal.
Los consumibles Magen están diseñados para ser compatibles con los sistemas automatizados de extracción más habituales, incluidas las plataformas de 32, 48 y 96 canales. Fabricados con polipropileno de alta calidad y verificados como libres de DNasa y RNasa, estos productos permiten una purificación fiable de ADN y ARN, al tiempo que minimizan el riesgo de contaminación.
Se ofrecen distintos formatos de placa y geometrías de pocillo para adaptarse a diversos requisitos del flujo de trabajo, como el procesamiento, el almacenamiento y la elución de las muestras. Los sistemas de peines de puntas facilitan la transferencia y el lavado eficaces de las partículas magnéticas durante los procedimientos automatizados de extracción.
La elección del sistema de preparación de ADN plasmídico depende principalmente de los requisitos del flujo de trabajo, más que del tipo de muestra. En la práctica de laboratorio, los factores clave suelen incluir el número de copias del plásmido, los requisitos relativos al contenido de endotoxinas, el volumen de cultivo y el tipo de procesamiento, ya sea manual, de alto rendimiento u orientado a la automatización.
Magen ofrece una gama estructurada de sistemas de extracción de ADN plasmídico basada en columnas y partículas magnéticas, que abarca tanto la preparación estándar de plásmidos como los flujos de trabajo con bajo contenido de endotoxinas para aplicaciones que requieren una mayor pureza. Los distintos productos de la serie están diseñados para diferentes volúmenes de cultivo y requisitos posteriores, desde preparaciones rutinarias a pequeña escala hasta el procesamiento de volúmenes mayores, el manejo de lotes en paralelo y los flujos de trabajo compatibles con la automatización.
Dentro de la gama actual de sistemas para plásmidos, el HiPure Plasmid Mini Kit (P1001) constituye el producto de referencia basado en columnas para la preparación rutinaria de plásmidos, mientras que el HiPure Plasmid EF Maxi Kit B (P1156B) es el principal modelo de referencia basado en columnas para flujos de trabajo EF a mayor escala y con requisitos más exigentes. Los demás productos de la serie amplían esta gama con opciones para una clarificación más rápida, preparaciones con bajo contenido de endotoxinas, procesamiento de alto rendimiento o purificación mediante partículas magnéticas.
Para seleccionar el producto según el flujo de trabajo, tanto para la preparación estándar de plásmidos como para aplicaciones con bajo contenido de endotoxinas, consulte la Guía de selección de kits de ADN plasmídico.