Introduction
MagPure Viral DNA/RNA Kit enables magnetic bead purification of viral nucleic acids from plasma, serum and other low background biological samples. In this workflow, viral particles are lysed and nucleic acids are captured by magnetic beads under optimized binding conditions.
The magnetic separation format allows rapid removal of contaminants through automated washing steps, making the system compatible with automated nucleic acid extraction instruments used in higher throughput laboratory environments.
Within the Magen Viral & Pathogen series, MagPure Viral DNA/RNA Kit represents the automated magnetic bead solution for routine viral nucleic acid preparation. Laboratories performing manual column purification may use HiPure Viral DNA/RNA Kit (IVD4175), while more complex samples can be processed using pathogen-focused systems such as MagPure Pathogen DNA/RNA Kit (IVD6672).
Details
Specifications
|
Features
|
Specifications
|
|
Main Functions
|
Extract viral DNA/RNA from 200μl cell-free samples by magnetic beads
|
|
Applications
|
RT-PCR，PCR，NGS
|
|
Products
|
Viral DNA / RNA
|
|
Purification method
|
Polydisperse magnetic beads
|
|
Purification technology
|
Magnetic beads technology
|
|
Process method
|
Manual or automatic
|
|
Sample type
|
cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant
|
|
Sample amount
|
200μl
|
Adaptive instrument
|
Nucleic acid extractor, pipetting workstation
|
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Technical Validation
MagPure Viral DNA/RNA Kit was evaluated using simulated low-input nucleic acid samples and virus-spiked porcine plasma to assess nucleic acid recovery, short-fragment DNA recovery and viral DNA/RNA extraction performance. The validation included 200 μL sample input, magnetic bead purification and elution volumes of 50–100 μL, reflecting the routine sample range used for serum, plasma and other low-cell-content biological samples.
In low-input DNA recovery testing, 25 ng of DL2000 DNA marker was added to RNase-free water and extracted with the kit. Qubit analysis showed total recovered DNA amounts of 20.9–22.1 ng, corresponding to recovery rates of 84–88% under the tested conditions. In a separate short-fragment recovery test using 250 bp DNA marker in porcine plasma or RNase-free water, the measured marker recovery ranged from 83.1% to 94.1%, supporting efficient recovery of short DNA fragments in both simple and plasma-based matrices.
RNA recovery was also evaluated using RNase-free water spiked with a defined RNA input. Nanodrop measurement showed recovered RNA amounts of 12.63–12.86 μg, corresponding to recovery rates of 90–92% under the tested conditions. These results support the kit’s ability to recover both DNA and RNA targets using the same magnetic bead purification workflow.
Functional viral extraction performance was tested by adding DNA virus (Hepatitis B Virus) and RNA virus (Newcastle Disease Virus) targets into porcine plasma followed by fluorescence quantitative PCR analysis. For the DNA virus model, Ct values increased from approximately 24.2 at 105 input level to approximately 32.4 at 103 input level. For the RNA virus model, Ct values increased from approximately 22.0–22.6 at 105 input level to approximately 32.2–32.9 at 103 input level. The Ct gradient across serial input levels indicates that the extracted viral nucleic acids were compatible with downstream qPCR / RT-qPCR detection.
Kit Contents
|
Contents
|
IVD5412
|
|
Purification Times
|
200 Preps
|
|
MagPure Particles N
|
5 ml
|
|
PK/Carrier RNA
|
50 mg
|
|
Protease Dissolve Buffer Blue
|
5 ml
|
Buffer MLB*
|
120 ml
|
Buffer MW1*
|
53 ml
|
RNase Free Water
|
30 ml
|
|
Cat.No
|
Reagent
|
IVD5412-F-96
|
|
PK/Carrier RNA
|
|
1x 24 mg/Bottle
|
|
Protease Dissolve Buffer Blue
|
|
1.8 ml/Bottle
|
|
Tip
|
|
1
|
|
Sample Plate (DW Plate)
|
500µl Buffer MLB
|
1
|
Wash 1 Plate (DW Plate)
|
500µl Buffer MW1
|
1
|
Wash 2 Plate (DW Plate)
|
500µl Buffer CW
|
1
|
Beads Plate (DW Plate)
|
500µl CW & 20µl MPN
|
1
|
Elute Plate (KF Plate)
|
90µl NFW
|
1
|
|
Cat.No
|
Reagent
|
IVD5412-TL-06
|
|
PK/Carrier RNA
|
|
24mg/310μg
|
|
Protease Dissolve Buffer Blue
|
|
1.8 ml
|
|
Tip
|
|
12
|
|
Prepackage Plate
(2.0ml Plate)
|
Row 1/7：500µl Buffer MLB
Row 2/8：500µl Buffer MW1
Row 3/9：500µl Buffer CW
Row 4/10：500µl Buffer CW&MPN
Row 5/11：100µl Buffer AVE
|
6
|
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
Experiment Data
Purchase Guide
For guidance on selecting the most appropriate viral or pathogen nucleic acid extraction system based on sample type, target nucleic acid, automation needs and downstream application:
👉 Viral & Pathogen Nucleic Acid Extraction Kit Selection Guide
For a broader technical overview of Magen viral / pathogen workflow routes, background-control logic and PCR, tNGS or mNGS application orientation:
👉 Magen Viral / Pathogen Workflows Explained
For detailed workflow notes covering representative pathogen extraction and enrichment routes:
👉 IVD6672 Pathogen DNA/RNA Extraction Workflow Note
👉 R6672B Low-Background Pathogen DNA/RNA Extraction Workflow Note
👉 R6672C Host-Background Reduction and Pathogen Enrichment Workflow Note
👉 IVD4179 Column-Based Pathogen Enrichment Workflow Note
