MagPure Circulating DNA Maxi Kit

MagPure Circulating DNA Maxi Kit (IVD5435) represents the magnetic bead configuration within the Magen circulating DNA portfolio. Developed through long-term refinement of magnetic particle chemistry, the system is designed for scalable plasma input ranging from 1 to 8mL.

Rather than focusing solely on yield claims, development emphasized bead surface consistency, adsorption stability and workflow adaptability. The extraction chemistry supports both manual magnetic stands and automation-oriented liquid handling systems.

In internal validation, plasma extraction results showed reproducible cfDNA yield across replicates. Comparative evaluations under equivalent conditions indicated performance consistent with established European magnetic systems. Stability testing across production batches demonstrated no measurable drift in physicochemical parameters over extended storage periods.

Laboratories preferring membrane-based workflows may refer to (HiPure Circulating DNA Kit – IVD3182). For lower plasma volumes or fragment-selective workflows, see:

· Small-volume magnetic extraction → (IVD5432)

· Size-selective enrichment → (12927)

· Column-based micro plasma extraction → (D3180)

IVD5435 serves as the scalable magnetic reference model within the system.

Workflow

Workflow Overview

The MagPure Circulating DNA Maxi Kit follows a magnetic bead–based workflow for purification of circulating DNA from plasma and serum. DNA is captured through particle–DNA interaction under mixing conditions rather than membrane flow-through, enabling flexible handling of larger sample volumes and low-abundance cfDNA.

Sample Handling Logic

This workflow supports 1–8 mL plasma or serum input. For lower input volumes, DNA binding is typically completed in a single step, while larger-volume samples are processed through staged binding to maintain recovery consistency. Magnetic separation replaces column flow-through, allowing scalable handling and compatibility with automated systems.

Time and Workflow Characteristics

Compared to column workflows, processing time is typically longer due to mixing, magnetic separation and repeated liquid handling steps. Under standard conditions, the workflow is generally completed within about 105–130 minutes. For detailed workflow steps, binding strategies and processing guidance, please refer to the Workflow Note in the Download section.

Engineering Characteristics

Magnetic Particle Consistency

Magnetic bead solid content and responsiveness are monitored during production. Experimental evaluation demonstrated that bead concentration directly influences recovery efficiency, and optimized bead-to-volume ratios are applied in the final workflow.

Plasma Recovery Stability

Across 4–8mL plasma processing, replicate extractions maintained stable Qubit concentration with minimal deviation, supporting higher-volume workflows.

Storage and Lot Stability

Physicochemical parameters (pH, density, conductivity) and marker recovery testing confirmed consistent performance across multiple production batches, including kits stored up to two years.

Workflow Scalability

IVD5435 integrates into a broader extraction system:

• Vacuum membrane workflow → (IVD3182)

• Small-volume magnetic kit → (IVD5432)

• Fragment-selective isolation → (12927)

Technical Validation

Marker Recovery Testing

Spike-in marker experiments demonstrated DNA recovery efficiencies typically above 80% across multiple reagent batches, confirming stable adsorption performance of the magnetic particle system.

Plasma cfDNA Extraction Performance

Extraction experiments using plasma samples showed circulating DNA yields typically around 5–7 ng per mL plasma when processing milliliter-scale plasma volumes.

Comparative Extraction Evaluation

Under equivalent extraction conditions, circulating DNA yields obtained using the MagPure system were comparable to those produced by widely used column-based circulating DNA extraction kits.

Kit Contents

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance.Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide

For a broader technical overview of cfDNA workflow routes, processing logic and downstream application orientation:

👉 cfDNA Extraction & Enrichment Workflows

For reference workflow structure and comparative processing logic across representative cfDNA workflows:

👉 cfDNA Workflow Notes (IVD3182 / IVD5435 / 12927)

Related cfDNA Application Notes: These application notes help explain how this circulating DNA extraction route fits different plasma cfDNA research workflows and downstream assay requirements.

👉 Application Map · Methylation Biomarkers · Prenatal cfDNA