Introduction

This Kit supports purification of circulating DNA from 1mL plasma samples using a silica membrane column workflow. It maintains consistent binding chemistry while reducing processing scale for moderate input volumes.

This configuration bridges micro-volume processing and larger vacuum-based workflows, offering flexibility for laboratories standardizing 1mL plasma inputs.

For higher plasma input volumes (1–5mL), refer to HiPure Circulating DNA Kit – IVD3182.

For magnetic bead-based workflows, see MagPure Circulating DNA Maxi Kit – IVD5435.

This product is for research use only.

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 1ml plasma, serum, body fluids
Applications	qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Serum, plasma and other cell-free fluid samples
Sample amount	1ml
Elution volume	≥30μl
Time per run	≤50 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris,pH 8.0).

Advantages

High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration - low elution volume, ensuring high nucleic acid concentration
High purity - low alcohol binding method, completely removing inhibitor and protein pollution
High recovery - DNA can be recovered at the level of PG by silica gel column purification

For detailed engineering characteristics and validation data, refer to the core circulating DNA extraction system.

Kit Contents

Contents	D318102	D318103
Purification Times	50 Preps	250 Preps
Buffer ACL	50 ml	250 ml
Buffer ACB*	60 ml	300 ml
Buffer DCW1*	22 ml	88 ml
Buffer DCW2*	10 ml	50 ml
Proteinase K	120 mg	540 mg
Protease Dissolve Buffer	10 ml	30 ml
Carrier RNA	110 μg	310 μg
Nuclease Free Water	10 ml	30 ml
HiPure CFDNA Mini Columns	50	250
2 ml Collection Tubes	100	500

Storage and Stability

Proteinase K and carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage(up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers shouldbe redissolved before use. Make sure that all buffers are at room temperature when used.

Purchase Guide

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide

For a broader technical overview of cfDNA workflow routes, processing logic and downstream application orientation:

👉 cfDNA Extraction & Enrichment Workflows

For reference workflow structure and comparative processing logic across representative cfDNA workflows:

👉 cfDNA Workflow Notes (IVD3182 / IVD5435 / 12927)

CAT NO	PRODUCT NAME	SIZE	PRICE
D318102	HiPure Circulating Nucleic acid Mini Kit	50 preps	$273.00
D318103	HiPure Circulating Nucleic acid Mini Kit	250 preps	$1,243.00

HiPure Circulating Nucleic acid Mini Kit

Introduction

Details

Principle

Advantages

Purchase Guide

Articles

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