Introduction
Blood samples contain multiple sources of DNA, including genomic DNA from leukocytes, mitochondrial DNA, circulating DNA released from apoptotic cells, and DNA originating from viruses or microorganisms present in blood. These nucleic acids are important targets for molecular diagnostics and are widely used in research workflows such as PCR amplification, genotyping analysis and HLA typing studies.
However, genomic DNA purification from blood samples presents several technical challenges. Blood is a potentially infectious biological material, which requires safe handling during sample preparation. In addition, blood contains complex components and inhibitory substances that may interfere with downstream molecular analysis. Traditional DNA extraction methods such as phenol–chloroform extraction or salting-out procedures typically require pre-processing steps to separate leukocytes by removing red blood cells. During this process, pathogens may remain active in the waste lysate, posing potential risks to laboratory operators and the environment. Furthermore, nucleic acids present outside leukocytes, including circulating DNA, viral DNA or microbial DNA, may be partially lost during red blood cell removal.
The HiPure Blood DNA Mini Kit (D311102) developed by Magen uses optimized silica membrane spin column purification technology that allows direct lysis of whole blood samples without the need for leukocyte separation. Whole blood is directly mixed with lysis buffer and protease, enabling efficient digestion of biological components while inactivating pathogens during the extraction process. This workflow simplifies sample preparation and improves laboratory safety.
The system supports purification from approximately 200–250μL anticoagulated whole blood and produces high-quality genomic DNA suitable for PCR amplification, genotyping workflows and HLA typing applications. The spin column format enables rapid purification using standard laboratory centrifuges and integrates easily into routine molecular biology workflows.
Within the Magen Blood DNA Extraction Systems, the HiPure Blood DNA Mini Kit represents the reference column-based workflow for genomic DNA purification from whole blood. Laboratories requiring automated extraction may use the MagPure Blood DNA Kit (D6311), while laboratories processing mixed biological samples may refer to the HiPure Universal DNA Kit (D3018).
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation total DNA from 200ul Whole Blood
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Applications
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PCR, southern bolt and virus detection, etc
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Purification method
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Mini spin column
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Purification technology
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Silica technology
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Process method
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Manual (centrifugation or vacuum)
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Sample type
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Whole Blood (fresh or frozen), serum, plasma, milk, saliva, and other liquid samples and cultured cells
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Sample amount
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<200μl whole blood or other liquid samples
<5*106 lymphocytes or Culture Cells
Non mammalian animals that have nucleus in red blood cells (rich in DNA, such as birds and fish) : 5~20μl whole blood at a time
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Elution volume
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≥20μl
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Time per run
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≤30 minutes
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Liquid carrying volume per column
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800µl
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Binding yield of column
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100µg
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Engineering Characteristics
Direct whole-blood lysis chemistry
The optimized lysis buffer enables efficient disruption of blood cells without pre-separation of leukocytes. This reduces workflow steps and minimizes potential DNA loss during sample processing.
Composite silica membrane adsorption
The column matrix is engineered for stable DNA binding capacity and reduced clogging when processing whole blood lysates. The membrane structure allows efficient adsorption of genomic DNA while proteins and cellular debris are removed during washing steps.
Optimized inhibitor removal
Blood-derived inhibitors such as hemoglobin and protein contaminants are effectively removed through the washing system, resulting in purified genomic DNA compatible with PCR and genotyping assays.
Standard centrifuge workflow
The spin-column format allows extraction using routine laboratory centrifuges without specialized instruments, supporting flexible laboratory workflows.
Technical Validation
Internal validation experiments using anticoagulated human whole blood samples demonstrated stable genomic DNA purification across multiple samples. Using approximately 250 μL blood, typical DNA yields ranged from 6–10 μg, with purity ratios A260/280 around 1.8–1.9, indicating high-quality genomic DNA suitable for downstream molecular applications.
Agarose gel electrophoresis analysis showed intact high-molecular-weight genomic DNA bands without detectable degradation, confirming suitability for PCR amplification and genotyping analysis.
Comparative testing against several commonly used commercial column-based DNA purification systems demonstrated comparable DNA yield and purity, supporting reliable performance in routine molecular biology workflows.
Typical Applications
• PCR amplification
• Genotyping analysis
• HLA typing studies
• Molecular diagnostics research
• Population genetics studies
• Sample preparation for sequencing workflows
Related Products
For laboratories processing different sample types or extraction workflows, the following systems are commonly used together with this kit:
• HiPure Universal DNA Kit (D3018) – purification of genomic DNA from mixed biological samples including tissue, blood, swabs and body fluids
• MagPure Blood DNA Kit (D6311) – magnetic bead-based automated purification of genomic DNA from whole blood
• MagPure Universal DNA Kit (IVD3102) – magnetic bead purification of genomic DNA from multiple biological sample types
• MagPure Blood RNA Kit (R6611) – automated purification of total RNA from anticoagulated blood samples
Kit Contents
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Contents
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D311102
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D311103
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Purification Times
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50
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250
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HiPure DNA Mini Columns I
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50
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2 x 125
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2ml Collection Tubes
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100
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5 x 100
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Buffer AL
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15 ml
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60 ml
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Buffer DW1
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30 ml
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150 ml
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Buffer GW2*
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12 ml
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50 ml
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Proteinase K
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24 mg
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120 mg
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Protease Dissolve Buffer
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1.8 ml
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10 ml
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Buffer AE
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15 ml
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60 ml
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Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
