The HiPure Viral DNA/RNA Kit is designed for rapid purification of viral nucleic acids from low cell-content biological samples such as plasma, serum, transport media and culture supernatants. The workflow is based on silica membrane adsorption under chaotropic binding conditions, allowing viral DNA and RNA molecules to be efficiently captured and purified through sequential washing and elution steps.

This system emphasizes streamlined processing for routine viral detection workflows where consistent nucleic acid recovery and rapid sample preparation are important. The protocol minimizes handling steps while maintaining compatibility with standard PCR and RT-PCR analysis.

Within the Magen Viral & Pathogen portfolio, HiPure Viral DNA/RNA Kit represents a column-based workflow optimized for routine viral nucleic acid extraction. Laboratories requiring automated processing may consider the magnetic bead–based MagPure Viral DNA/RNA Kit (IVD5412), while sequencing-oriented pathogen workflows are supported by HiPure Pathogen DNA/RNA Kit (IVD4179).

Specifications

Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).

Advantages

• Fast - column purification without PK digestion
• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Safe - no phenol chloroform extraction required
• Sensitive - DNA/RNA can be recovered at the level of PG

Kit Contents

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide