Introduction

The MagPure Blood RNA Kit C is optimized for RNA purification from low-input blood samples using magnetic bead technology. The workflow supports extraction of total RNA including microRNA from small sample volumes such as whole blood, buffy coat or cultured cells.

RNA molecules are captured by high-affinity magnetic particles and purified through multiple washing steps before elution. The system supports automated RNA purification workflows and is suitable for microRNA expression profiling or small-volume blood RNA studies.

For routine automated blood RNA purification from standard sample volumes laboratories may use the MagPure Blood RNA Kit (R6611).

Details

Principle

The Kit can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤ 1 x10⁶ cells suspension, 50 μl Whole Blood, 50μl buffy coat, 20μl bone marrow. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. RNA/DNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were digested using DNase and washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and

Kit Contents

Contents	R661101C	R661102C	R661103C
Purification times	48 Preps	96 Preps	480 preps
MagPure RNA Particles	1.0 ml	1.5 ml	6 ml
Proteinase K	24 mg	48 mg	220 mg
Protease Dissolve Buffer	1.8 ml	1.8 ml	15 ml
DNase I	600 μl	2 x 600 μl	10 x 600 µl
DNase Buffer	15 ml	15 ml	60 ml
Buffer AL	10 ml	10 ml	60 ml
Buffer MCB*	9 ml	9 ml	30 ml
Buffer MW1*	13 ml	22 ml	110 ml
Buffer MW2*	6 ml	20 ml	100 ml
RNase Free Water	10 ml	15 ml	120 ml

Storage and Stability

MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.

Purchase Guide

Choosing the appropriate RNA extraction system depends on sample type, RNA species and workflow requirements.

For detailed selection guidance across blood and liquid RNA workflows, refer to the Blood RNA Kits Selection Guide.

Articles

Download

R6611C MagPure Blood RNA Kit C(with DNase I).pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
R661101C	MagPure Blood RNA Kit	48 preps	$286.00
R661102C	MagPure Blood RNA Kit	96 preps	$525.00
R661103C	MagPure Blood RNA Kit	480 preps	$2,100.00

MagPure Blood RNA Kit C

Introduction

Details

Principle

Purchase Guide

Articles

Download

Related Products

Popular products

HiPure Bone DNA Kit

HiPure Plant RNA Plus Kit

MagPure Forensic DNA Kit

MagPure Pathogen DNA/RNA Enrich Kit

MagPure Universal DNA Kit

HiPure Total RNA Plus Kit

MagPure FFPE DNA/RNA Kit

HiPure FFPE DNA Kit

MagPure Circulating DNA Maxi Kit

HiPure Circulating DNA Kit

HiPure Blood DNA Mini Kit