RNA extraction from blood and liquid samples presents unique technical challenges due to sample complexity, low RNA abundance, and the presence of inhibitors or degradation factors. Different workflows are required depending on whether the target is leukocyte RNA, circulating RNA, or microRNA from cell-free body fluids.
Magen provides a structured portfolio of blood and liquid RNA extraction systems based on silica membrane, magnetic bead, and phenol-based chemistries. These systems support both manual and automated workflows and are optimized for different sample types including whole blood, stabilized blood samples, serum, plasma and other body fluids.
This guide is designed to help laboratories and distributors select appropriate RNA extraction solutions based on sample type, input format and downstream application requirements such as RT-PCR, gene expression analysis and microRNA profiling.
| If your sample or experiment requires | Recommended Kit |
| Routine RNA extraction from whole blood | HiPure Blood RNA Mini Kit (R4161) |
| RNA extraction from stabilized blood samples (PAXgene tubes) | HiPure Paxgene Blood RNA Kit (R4168) |
| Automated RNA purification from blood samples | MagPure Blood RNA Kit (R6611）⭐ |
| Low-input automated blood RNA extraction or microRNA analysis | MagPure Blood RNA Kit C (R6611C) |
| RNA extraction from frozen blood or biobank samples | MagPure Blood RNA Kit II (R6613) |
| Maximum RNA and microRNA recovery from serum or plasma | HiPure Liquid RNA (miRNA) Kit (R4163) |
| Rapid serum/plasma miRNA extraction without phenol | HiPure Serum miRNA Kit (R4314) |
| Automated or high-throughput serum miRNA purification using magnetic bead workflow | MagPure Serum miRNA Kit (R6628) |
| Product | Technology | RNA Type | Sample Type | Typical Input | Key Strategy | Best For |
| HiPure Blood RNA Mini Kit (R4161) | Spin column | Total RNA (>200 nt) | Whole blood | 1-1.5 ml | Erythrocyte lysis followed by leukocyte RNA purification | Routine blood RNA extraction for RT-PCR, qPCR and gene expression analysis |
| HiPure Paxgene Blood RNA Kit (R4168) | Spin column | Total RNA | Stabilized blood samples in paxgene tube | 2.5 ml PAXgene tube equivalent | RNA purification compatible with blood RNA stabilization tubes | Gene expression analysis from stabilized blood samples including clinical transcriptomics and biomarker studies |
| MagPure Blood RNA Kit (R6611）⭐ | Magnetic bead | Total RNA | Whole blood / buffy coat / bone marrow | 1-1.5 ml | RBC lysis followed by magnetic RNA purification with DNase digestion | Automated blood RNA purification for RT-PCR, NGS and viral RNA detection |
| MagPure Blood RNA Kit C (R6611C) | Magnetic bead | Total RNA + miRNA | Whole blood / buffy coat | 50 µl | Magnetic bead purification optimized for low-input blood RNA including microRNA | Blood microRNA profiling and small RNA analysis |
| MagPure Blood RNA Kit II (R6613) | MagZol + Magnetic bead | Total RNA | Fresh or frozen blood / buffy coat / bone marrow | 1 ml | MagZol-based lysis enabling RNA extraction directly from frozen blood samples | Biobank samples, frozen blood processing and RNA analysis after long-term storage or transport |
| HiPure Liquid RNA (miRNA) Kit (R4163) | MagZol + spin column | Total RNA + miRNA | Serum / plasma / body fluids | 250 µl | Phenol–guanidine lysis with silica column purification | Maximum RNA and microRNA recovery from liquid samples for sensitive expression profiling |
| HiPure Serum miRNA Kit (R4314) | Spin column | Total RNA + miRNA | Serum / plasma | 300–900 µl | Heavy metal ion–based protein precipitation combined with silica membrane RNA purification | Rapid phenol-free microRNA extraction from serum and plasma for RT-PCR, Northern blot and miRNA expression analysis |
| MagPure Serum miRNA Kit (R6628) | Magnetic bead | Total RNA + miRNA | Serum / plasma | 300–500 µl | Heavy metal ion–based protein precipitation followed by magnetic bead RNA purification | Automated or high-throughput microRNA purification from serum and plasma for RT-PCR and miRNA expression analysis |
For detailed product specifications and workflow descriptions, please refer to individual product pages.
