Spin column selection is primarily determined by sample input volume, expected nucleic acid yield, and downstream workflow requirements. Different column formats are designed to balance binding capacity, flow characteristics, and sample compatibility across various laboratory workflows.
· Small-volume samples → Micro column
· Routine sample input → Mini column
· High input samples → Midi / Maxi column
· High-throughput processing → DNA Plate
The table below provides a structured comparison of column formats to support rapid selection based on sample input and workflow requirements.
| Cat. No. | Product Name |
Membrane Type & Layers |
Collection Tube |
Plasmid DNA Binding Capacity |
gDNA/RNA Binding Capacity |
Minimum Elution Volume |
Maximum Sample Volume |
|
C13010 |
HiPure DNA Nano Column | 2-layer GF/F |
2ml without cap |
5μg |
20μg |
10μl | 700μl |
|
C13011 |
HiPure DNA Micro Column | 3-layer GF/F |
2ml without cap |
10μg |
50μg |
15μl | 700μl |
|
C13100 |
HiPure DNA Mini Column I | 2-layer GF/B |
2ml without cap |
15μg |
100μg |
30μl | 700μl |
|
C13110 |
HiPure DNA Mini Column II | 4-layer GF/B |
2ml without cap |
35μg |
200μg |
50μl | 800μl |
|
C13111 |
HiPure RNA Mini Column | 3-layer GF/B |
2ml without cap |
30μg |
200μg |
30μl | 800μl |
|
C13112 |
HiPure Viral Mini Column | 3-layer GF/F |
2ml without cap |
30μg |
200μg |
30μl | 800μl |
|
C13113 |
HiPure CFDNA Mini Column | 3-layer GF/F,1-layer GF/B |
2ml without cap |
30μg |
200μg |
30μl | 800μl |
|
C13120 |
HiPure DNA Midi Column | 4-layer GF/B |
15ml Centrifuge tube |
125μg |
1mg |
500μl | 4ml |
|
C13121 |
HiPure DNA Midi Column III | 8-layer GF/B |
15ml Centrifuge tube |
250μg |
1mg |
500μl | 4ml |
|
C13122 |
HiPure DNA Maxi Column | 4-layer GF/B |
50ml Centrifuge tube |
500μg |
5mg |
1000μl | 20ml |
|
C13123 |
HiPure DNA Maxi Column III |
8-layer GF/B |
50ml Centrifuge tube |
1mg | 5mg | 1000μl | 20ml |
|
C13124 |
HiPure DNA Maxi Column C |
8-layer GF/B |
50ml high speed Centrifuge tube |
1mg |
5mg |
700μl | 12ml |
|
C13130 |
2-layer GF/F |
1.6ml Plate |
30μg |
100μg |
80μl |
900μl |
|
|
C13131 |
2-layer GF/B |
1.6ml Plate |
30μg |
100μg |
80μl |
900μl |
Note: GF/F and GF/B represent different glassfiber membrane structures used to balance binding efficiency and flow performance. GF/F membranes (~0.7 μm) are typically used for low-input or fragmented nucleic acid workflows, while GF/B membranes (~1.0 μm) are applied in routine and high-capacity purification.
