Spin Column for Circulating DNA Purification

Introduction

This column is configured for circulating DNA workflows where sample volume is larger and nucleic acid concentration is low. It is typically used in plasma or serum processing where recovery efficiency across milliliter-scale inputs becomes critical.

The column can be used with extended loading configurations, allowing gradual binding of fragmented DNA without compromising membrane stability.

C13114 HiPure cfDNA Mini Column Set I (Centrifuge Method)

C13115 HiPure cfDNA Column Set Ⅱ (Vacuum Method)

Details

Specifications

Features	Specifications
Recommended application	Circulating or viral nucleic acid isolation from large volumes of cell free samples (1-5ml)
Preservation conditions	Room temperature
Stability	Up to 4 years
Filter membrane	High quality glass fiber filter GF/F, 4 layers (3 x GF/F, 1 x GF/B)
Membrane aperture	3 x 0.7μm, 1 x 1.0μm
Maximum binding yield of plasmid	30 μg
Maximum yield of alcohol mediated Binding	200 μg
Single liquid carrying capacity of column	800 μl
Minimum elution volume	30 μl
Withstand centrifugal force	16,000 x g
Centrifuge	Small high speed centrifuge (2ml)

Adsorption Mechanism

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.

Ordering information

CAT.No.	Product Name	Package
C13113	HiPure cfDNA Mini Column (3 x GF/F, 1 x GF/B)	100/Bag
C13114	HiPure cfDNA Mini Column (3 x GF/F, 1 x GF/B) with 50ml Centrifuge Tube, Extender Tube, Collection Tube, Collection Tube Ⅲ	100/Pack
C13115	HiPure cfDNA Mini Column (3 x GF/F, 1 x GF/B) with Collection Tubes, Extender Tube, Vac-Connector	100/Pack
C13301	Vac connector	100/Bag
C13302	Extender Tube	50/Bag

Purchase Guide

HiPure cfDNA Column (C13113) is designed for purification of circulating DNA from plasma and other cell-free biological samples, with optimized binding conditions for short DNA fragments.

For viral nucleic acid extraction workflows, HiPure Viral Column (C13112) may be considered.

For routine genomic DNA purification, HiPure DNA Mini Column (C13100) is generally more suitable.

For a structured comparison of column formats:

👉 Spin Column Selection Guide

CAT NO	PRODUCT NAME	SIZE	PRICE
C13113	HiPure cfDNA Mini Column	100/Bag	$156.00
C13114	HiPure cfDNA Column Set I (Centrifuge Method)	100/Pack	$600.00
C13115	HiPure cfDNA Column Set Ⅱ (Vacuum Method)	100/Pack	$600.00

HiPure cfDNA Column Set

Introduction

Details

Purchase Guide

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