| CAT NO | PRODUCT NAME | SIZE | PRICE |
| C13123 | HiPure DNA Maxi Column III | 100/Bag | $884.00 |
This column is used for plasmid preparation at the largest scale within the column system. It provides sufficient binding capacity to support high-yield plasmid workflows from bulk cultures.
Compared with midi formats, it is better suited for applications where DNA yield is prioritized over processing speed.
Specifications
| Features | Specifications |
| Recommended application | Plasmid Maxi preparation |
| Preservation conditions | Room temperature |
| Stability | Up to 4 years |
| Filter membrane | High quality glass fiber filter GF/B, 8 layers |
| Membrane aperture | 1.0 μm |
| Maximum binding yield of plasmid | 1 mg |
|
Maximum yield of alcohol mediated binding |
5 mg |
|
Plasmid yield |
Up to 1mg |
|
Single liquid carrying capacity of column |
20 ml |
|
Minimum elution volume |
800 μl |
|
Withstand centrifugal force |
3,000-5,000 x g |
|
Centrifuge |
Low speed centrifuge for 50ml centrifuge tubes, >3000 x g, swing-out Rotor |
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
| CAT.No. |
Product Name |
Package |
| C13123 |
HiPure DNA Maxi Column III (8 x GF/B) with 50ml Collection Tubes |
100/Bag |
HiPure DNA Maxi Column III (C13123) is designed for large-scale DNA purification with high sample input and maximum yield requirements.
For routine workflows, HiPure DNA Mini Column (C13100) offers a more efficient balance of throughput and reagent consumption.
For intermediate-scale DNA preparation, HiPure DNA Midi Column (C13120) may be considered.
For selection guidance across different column capacities:
D6381
D3141F
D3124
R4150
D6359D
R6672C
IVD4179
R4165
IVD3102
D3018
IVD5412
IVD4175
R6611
R4310
IVD3020
R4111
R6327
D6364
D3142
D3146
D6351
D3187
D3126
IVD5435
IVD3182
D6311
D3111
