Introduction

The HiPure Tissue & Blood DNA Maxi Kit extends the column-based genomic DNA purification workflow to large sample volumes. The Maxi column format allows processing of substantially larger biological materials while maintaining the same silica membrane adsorption chemistry used in standard HiPure DNA purification systems.

The kit supports purification of genomic DNA from up to 10 ml whole blood or approximately 1 g tissue, enabling preparation of high DNA yields required for large-scale genomic studies, reference DNA preparation, or archival DNA storage.

Within the Magen DNA extraction systems this kit represents a large-volume column workflow. Laboratories performing routine DNA isolation may use the HiPure Universal DNA Kit (D3018), while automated high-throughput laboratories may adopt the MagPure Universal DNA Kit (IVD3102).

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 10ml blood and 1g tissue using Maxi column
Applications	PCR, southern bolt and virus detection, etc
Purification method	Maxi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount	3-10ml
Elution volume	≥700μl
Time per run	≤90 minutes
Liquid carrying volume per column	4ml
Binding yield of column	5mg

Principle

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast - without separation of leukocytes, organic extraction or ethanol precipitation
Simple - all nucleic acids can be obtained by direct digestion
Pertinence - specially designed for isolating DNA from 3-10ml blood and related body fluids
Wide applicability - handle a variety of liquid samples

Kit Contents

Contents	D311502	D311503
Purification Times	10	50
HiPure gDNA Maxi Columns	10	50
50ml Collection Tubes	20	100
Buffer ATL	120 ml	550 ml
Buffer AL	120 ml	2 x 300 ml
Buffer GW1*	53 ml	220 ml
Buffer GW2*	25 ml	110 ml
RNase A	40 mg	180 ml
Proteinase K	120 mg	540 mg
Protease Dissolve Buffer	12 ml	50 ml
Buffer AE	30 ml	120 ml

Storage and Stability

Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide

CAT NO	PRODUCT NAME	SIZE	PRICE
D311502	HiPure Tissue&Blood DNA Maxi Kit	10 preps	$174.00
D311503	HiPure Tissue&Blood DNA Maxi Kit	50 preps	$622.00

HiPure Tissue&Blood DNA Maxi Kit

Introduction

Details

Principle

Advantages

Kit Contents

Purchase Guide

Articles

Download

Related Products

MagPure Universal DNA Kit

HiPure Universal DNA Kit

HiPure Tissue&Blood DNA Midi Kit

Popular products

MagPure Universal HW DNA Kit

HiPure Stool DNA Mini Kit

HiPure Bone DNA Kit

HiPure Plant RNA Plus Kit

MagPure Forensic DNA Kit

MagPure Pathogen DNA/RNA Enrich Kit

HiPure Pathogen DNA/RNA Kit (NGS)

HiPure HP Plant RNA Mini Kit

MagPure Universal DNA Kit

HiPure Universal DNA Kit

MagPure Viral DNA/RNA Kit

HiPure Viral DNA/RNA Kit

MagPure Blood RNA Kit

HiPure Universal miRNA Kit

MagPure Universal RNA Kit

HiPure Total RNA Plus Kit

MagPure FFPE DNA/RNA Kit

MagPure Stool DNA Kit

HiPure Soil DNA Kit

HiPure Bacterial DNA Kit

MagPure Plant DNA Kit

HiPure HP Plant DNA Kit

HiPure FFPE DNA Kit

MagPure Circulating DNA Maxi Kit

HiPure Circulating DNA Kit

MagPure Blood DNA Kit

HiPure Blood DNA Mini Kit