| CAT NO | PRODUCT NAME | SIZE | PRICE |
| C13100 | HiPure DNA Mini Column I | 1000/Bag | $320.00 |
This column represents the standard format for routine nucleic acid purification in laboratory workflows. It is typically used for genomic DNA extraction, RNA purification, and cleanup steps following enzymatic reactions.
The membrane structure is optimized for consistent binding performance across a wide range of sample types, making it suitable for general-purpose use without the need for workflow adjustments.
Specifications
| Features | Specifications |
| Recommended application | gDNA extraction, RNA extraction, DNA/RNA clean up |
| Preservation conditions | Room temperature |
| Stability | Up to 4 years |
| Filter membrane | High quality glass fiber filter GF/B, 2 layers |
| Membrane aperture | 1.0μm |
| Maximum binding yield of plasmid | 15 μg |
|
Maximum yield of alcohol mediated Binding |
100 μg |
|
Single liquid carrying capacity of column |
700 μl |
|
Minimum elution volume |
30 μl |
|
Withstand centrifugal force |
12,000 x g |
|
Centrifuge |
Small high speed centrifuge (2ml) |
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
| CAT.No. |
Product Name |
Package |
| C13100 |
HiPure DNA Mini Column I (2 x GF/B) with 2ml Collection Tubes |
1000/Bag |
HiPure DNA Mini Column (C13100) is designed for routine DNA purification across a wide range of biological samples and laboratory workflows.
For low-input or highly diluted samples, HiPure DNA Nano Column (C13010) or HiPure DNA Micro Column (C13011) may improve recovery efficiency.
For larger sample volumes or higher DNA yield requirements, HiPure DNA Midi Column (C13120) or HiPure DNA Maxi Column (C13122) can be considered.
For full comparison across column formats:
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