The HiPure Blood RNA Kit provides a column-based workflow for purification of total RNA from whole blood samples. The protocol includes erythrocyte lysis followed by isolation of leukocytes prior to RNA purification, allowing recovery of cellular RNA from peripheral blood samples.

Following leukocyte enrichment, RNA molecules are purified using a silica membrane column and sequential washing steps to remove proteins and salts. The workflow supports routine gene expression analysis and RT-PCR applications using blood-derived RNA.

For automated RNA purification from blood samples laboratories may use the MagPure Blood RNA Kit (R6611), while low-input blood RNA extraction including microRNA analysis can be performed using the MagPure Blood RNA Kit C (R6611C).

Specifications

Principle

The Kit simplifies isolation of RNA from blood with a fast spin-column procedure. Red blood cells are selectively lysed and white cells collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the DNA spin column, the sample is applied to the RNA column. Total RNA binds to the membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100µl RNase-free water (provided with the kit) for direct use in any downstream application.

Advantages

• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast - isolation of several samples can be completed in 20 minutes by using column purification method
• Sensitive - RNA can be purified at the level of PG

Kit Contents

Storage and Stability

HiPure Blood RNA Mini Kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the RTL Lysis Buffer. Dissolve by warming buffer to 37°C.

Choosing the appropriate RNA extraction system depends on sample type, RNA species and workflow requirements.

For detailed selection guidance across blood and liquid RNA workflows, refer to the Blood RNA Kits Selection Guide.