The HiPure Liquid RNA (miRNA) Kit enables purification of total RNA including microRNA from serum, plasma and other biological fluids. The workflow combines MagZol phenol–guanidine lysis with silica membrane purification to improve RNA recovery from liquid samples with low RNA abundance.

Following MagZol lysis, RNA molecules bind to a silica membrane column and are purified through sequential washing steps before elution. The system supports microRNA profiling, RT-PCR analysis and other applications requiring sensitive RNA detection from liquid samples.

For microRNA enrichment workflows laboratories may use the HiPure Universal miRNA Kit (R4310).

Specifications

Principle

0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100 µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.

Advantages

• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast – isolation of several samples can be completed in 40 minutes by using column purification method
• Safety - no phenol chloroform extraction required
• Sensitive - direct lysis of blood, plasma and other samples without separation of leukocytes

Kit Contents

Storage and Stability

MagZol LS Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Choosing the appropriate RNA extraction system depends on sample type, RNA species and workflow requirements.

For detailed selection guidance across blood and liquid RNA workflows, refer to the Blood RNA Kits Selection Guide.