Introduction

The HiPure Serum miRNA Kit provides a column-based workflow for purification of cell-free RNA including microRNA from serum, plasma, urine and secretion samples. The protocol uses chaotropic lysis followed by selective protein precipitation and silica membrane purification.

The workflow enables rapid microRNA extraction from cell-free body fluids without phenol-based reagents and supports routine RT-PCR analysis and microRNA detection workflows.

For automated serum microRNA purification workflows laboratories may refer to the MagPure Serum miRNA Kit (R6628), while enrichment-based miRNA purification workflows are supported by the HiPure Universal miRNA Kit (R4310).

Details

Specifications

Features	Specifications
Main Functions	Isolation cell-free RNA and miRNA from 0.3-0.9mL cell-free body fluids such as serum, plasma, urine and secretion samples
Applications	RT-PCR, Northern blotting, poly(A)+ RNA purification, nuclease protection assays, in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	cell-free body fluids such as serum, plasma, urine and secretion samples
Sample amount	0.3–0.9 mL
Minimum Elution Volume	20 µL
Time per run	Within 45 minutes
Maximum Column Loading Volume	800 µL
Maximum Nucleic Acid Binding Capacity	100 µg

Principle

HiPure RNA technology simplifies total RNA isolation. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After the removal of protein the binding conditions for nucleic acids are adjusted by adding isopropanol. Total nucleic acids are bound to the column. Optionally, DNA can be removed by an on-column DNase digest. The remaining nucleic acids are washed and eluted with minimal amounts of RNase-Free water.

Kit Contents

Contents	R431402	R431403
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	100	2 x 250
2ml Collection Tubes	100	2 x 250
Buffer CFL	6 ml	30 ml
Buffer CFP	1.8 ml	10 ml
Buffer RWC	20 ml	80 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	60 ml

Storage and Stability

The kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Choosing the appropriate RNA extraction system depends on sample type, RNA species and workflow requirements.

For detailed selection guidance across blood and liquid RNA workflows, refer to the Blood RNA Kits Selection Guide.

CAT NO	PRODUCT NAME	SIZE	PRICE
R431402	HiPure Serum miRNA Kit	50 preps	$149.00
R431403	HiPure Serum miRNA Kit	250 preps	$646.00

HiPure Serum miRNA Kit

Introduction

Details

Principle

Purchase Guide

Articles

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