Introduction

The MagPure Serum miRNA Kit uses magnetic particle adsorption technology to enable automated purification of microRNA from serum and plasma samples. RNA molecules are captured by magnetic particles and purified through sequential washing steps prior to elution.

The workflow supports automated extraction systems and is suitable for laboratories performing high-throughput microRNA purification from liquid samples.

For column-based serum microRNA extraction workflows laboratories may refer to the HiPure Serum miRNA Kit (R4314).

Details

Specifications

Features	Specifications
Main Functions	Isolation miRNA from 0.3-0.5ml serum or plasma using magnetic particles
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Adaptive instrument	Nucleic acid extractor, pipetting workstation
Sample type	Serum, plasma, acellular samples
Sample amount	300μl

Principle

This product is based on the purification method of high binding magnetic particles. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.

Advantages

Safe - no phenol chloroform extraction and no use of Trizol reagent
Fast - several samples can be extracted in 60 minutes by column method

Kit Contents

Contents	R662801	R662802	R662803
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagPure Particles N	1.7 ml	3.5 ml	17 ml
Buffer CFL	6 ml	12 ml	60 ml
Buffer CPL	1.8 ml	3.5 ml	20 ml
Buffer MGW1*	30 ml	60 ml	250 ml
Buffer MW2*	12 ml	25 ml	100 ml
RNase Free Water	10 ml	20 ml	60 ml

Storage and Stability

MagPure Particle N should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Choosing the appropriate RNA extraction system depends on sample type, RNA species and workflow requirements.

For detailed selection guidance across blood and liquid RNA workflows, refer to the Blood RNA Kits Selection Guide.

Articles

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R6628 MagPure Serum miRNA Kit.pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
R662801	MagPure Serum miRNA Kit	48 preps	$224.00
R662802	MagPure Serum miRNA Kit	96 preps	$323.00
R662803	MagPure Serum miRNA Kit	5 x 96 preps	$1,193.00

MagPure Serum miRNA Kit

Introduction

Details

Principle

Purchase Guide

Articles

Download

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