HiPure Pathogen DNA/RNA Kit (NGS)

The HiPure Pathogen DNA/RNA Kit is designed for purification of pathogen nucleic acids from complex biological samples including blood, sputum and tissue homogenates. The workflow integrates chemical lysis with bead-assisted microbial disruption to improve release of nucleic acids from bacterial and fungal cells.

Following lysis, DNase treatment reduces background host DNA before nucleic acids are purified using a silica column. Purified pathogen DNA and RNA are recovered through sequential washing and elution steps.

This system is frequently used in pathogen sequencing workflows where recovery of microbial nucleic acids from complex clinical matrices is required. For magnetic bead–based pathogen extraction, laboratories may use MagPure Pathogen DNA/RNA Kit (IVD6672), while host background reduction prior to sequencing can be achieved using MagPure Pathogen DNA/RNA Enrich Kit (R6672C).

Specifications

Extraction Principle

Pathogen detection in complex clinical samples often requires efficient disruption of microbial cells while minimizing interference from host nucleic acids.

The HiPure Pathogen DNA/RNA Kit combines chemical lysis and bead-assisted mechanical disruption to release nucleic acids from microorganisms. DNase treatment is applied during the extraction workflow to reduce background host DNA prior to nucleic acid purification.

Released nucleic acids bind to the silica membrane under chaotropic binding conditions and are subsequently purified through washing and elution steps.

Engineering Features

The workflow integrates bead-assisted microbial lysis with column purification to improve nucleic acid recovery from complex biological matrices such as blood, sputum or tissue homogenates. The column chemistry is optimized for fragmented nucleic acids and supports stable purification of pathogen DNA and RNA from samples with variable microbial content.

Kit Contents

Storage and Stability

Proteinase K and DNase I (Powder) should be stored at 2–8°C upon arrival. However, short-term storage (DNase I up to 1 week, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affecttheir performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide