The HiPure Universal RNA Kit combines MagZol reagent lysis with silica membrane purification to enable efficient RNA extraction from difficult or heterogeneous tissue samples. The MagZol-based phenol–guanidine chemistry improves disruption of fibrous tissues, lipid-rich tissues and other challenging biological materials.

Following MagZol lysis, RNA is captured on a silica membrane column and purified through multiple washing steps to remove proteins, genomic DNA residues and organic contaminants. The workflow is suitable for tissue samples where conventional column-based RNA extraction may show reduced yield or purity.

For routine RNA purification workflows laboratories may use the HiPure Total RNA Plus Kit (R4111), while automated magnetic bead RNA purification is supported by the MagPure Universal RNA Kit (IVD3020).

Specifications

Principle

Tissue samples (10-100mg) are homogenized in MagZol Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.

Advantages

• Efficient DNA removal - one step RNA extraction can effectively remove genomic DNA
• High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast - several samples can be extracted in 30 minutes
• High applicability - samples including animals, plants, bacteria, cells, etc.
• High output - enable to process large samples and the yield can be up to 200μg

Kit Contents

Storage and Stability

MagZol Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

Select the right purification kit to get impactful results：

Ⅰ. Purchase guide of Magen kits based on sample

Ⅱ. Cross purchase guide for Magen kits vs Other brands

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